caa a22 4500 606233652 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2374-6 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2374-6 Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats [Elektronische Daten] [Jayanthy Govindaraj, Subramanian Sorimuthu Pillai] Persistent hyperglycemia and elevated levels of free fatty acids (FFA) contribute to oxidative stress, a proximate cause for the onset and progression of diabetes and its complications. The present study was hypothesized to evaluate the anti-diabetic potential of Rosmarinic acid (RA) during high-fat diet (HFD)—streptozotocin (STZ)-induced type 2 Diabetes (T2D) in wistar albino rats. Oral administration of RA (100mg/kg b.w) significantly (p<0.05) increased the insulin sensitivity index (ISI0,120), while the levels of blood glucose, HbA1c, advanced glycation end products (AGE), TNF-α, IL-1β, IL 6, NO, p-JNK, P38 MAPK and NF-κB were significantly reduced, with a concomitant elevation in the plasma insulin levels in diabetic rats. Furthermore, RA treatment significantly (p<0.05) reduced the levels of triglycerides, FFA and cholesterol in serum, and reduced the levels of lipid peroxides, AOPP's and protein carbonyls in the plasma and pancreas of diabetic rats. The diminished activities of pancreatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) and the decreased levels of plasma ceruloplasmin, vitamin C, vitamin E and reduced glutathione (GSH) in diabetic rats were also significantly (p<0.05) recovered upon RA treatment denoting its antioxidant potential which was confirmed by Nrf-2, hemeoxyenase (HO-1) levels. Histological, ultrastructural and immunohistochemical data demonstrate that oral administration of RA protects pancreatic β-cells from oxidative niche in HFD-STZ-induced experimental diabetes. Our findings suggest that the oral treatment with RA alleviates pancreatic β-cell dysfunction and glucolipotoxicity-mediated oxidative stress during HFD-STZ-induced T2DM, perhaps through its antioxidant potential. Springer Science+Business Media New York, 2015 Rosmarinic acid nationallicence Cytokines nationallicence NF-κB nationallicence P38 MAPK nationallicence p-JNK nationallicence Nrf-2 nationallicence Govindaraj Jayanthy Department of Biochemistry, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Sorimuthu Pillai Subramanian Department of Biochemistry, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 143-159 0300-8177 404:1-2<143 2015 404 11010 https://doi.org/10.1007/s11010-015-2374-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2374-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Govindaraj Jayanthy Department of Biochemistry, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 700 1- Sorimuthu Pillai Subramanian Department of Biochemistry, University of Madras, Guindy Campus, 600 025, Chennai, Tamilnadu, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 143-159 0300-8177 404:1-2<143 2015 404 11010