caa a22 4500 606233709 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2378-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2378-2 Antioxidant capacity and structural changes of human serum albumin from patients in advanced stages of diabetic nephropathy and the effect of the dialysis [Elektronische Daten] [Marisol Rosas-Díaz, Menandro Camarillo-Cadena, Andrés Hernández-Arana, Eva Ramón-Gallegos, Rafael Medina-Navarro] Changes in the antioxidant capacity of albumin and alterations of the albumin structural conformation were examined in patients in advanced stages of diabetes nephropathy. Human serum albumin was purified from diabetic patients in pre-dialysis (glomerular filtration rate [GFR] between 15 and 29mlmin−11.73m−2) and those in dialysis (GFR≤15mlmin−11.73m−2) and then compared with albumin from patients with a normal GFR (>90mlmin−1m−2). We evaluated the antioxidant capacity of albumin using an enhanced chemiluminescence-based assay and thiol group content, and the structural changes were evaluated by circular dichroism and fluorescence spectroscopy. The antioxidant capacity and thiol content of albumin from patients in advanced stages of diabetic nephropathy were markedly reduced. The circular dichroism spectra showed a mean albumin α-helix content reduction from 44 to 37% and from 44 to 30% between the control group and pre-dialysis and dialysis patients, respectively. Additionally, the fluorescence intensity was reduced by 4.2 and 13% for the groups 4 and 5, respectively, in relation with the control. These data provide evidence for the partial denaturation of albumin and exacerbated oxidative stress among patients in advanced stages of diabetes nephropathy before and even after dialysis. Springer Science+Business Media New York, 2015 Human serum albumin nationallicence Albumin conformational changes nationallicence Diabetes nationallicence Diabetic nephropathy nationallicence Hemodialysis nationallicence Oxidative stress nationallicence Rosas-Díaz Marisol Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Camino de la Arboleda 300, Ex Hacienda de San José La Huerta., C.P. 58341, Morelia, Michoacán, Mexico aut Camarillo-Cadena Menandro Department of Physical Chemistry, Univesidad Autónoma Metropolitana de Iztapalapa (UAM-Iztapalapa), Mexico, DF, Mexico aut Hernández-Arana Andrés Department of Physical Chemistry, Univesidad Autónoma Metropolitana de Iztapalapa (UAM-Iztapalapa), Mexico, DF, Mexico aut Ramón-Gallegos Eva Environmental Cytopathology Laboratory, ENCB-IPN, Mexico, DF, Mexico aut Medina-Navarro Rafael Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Camino de la Arboleda 300, Ex Hacienda de San José La Huerta., C.P. 58341, Morelia, Michoacán, Mexico aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 193-201 0300-8177 404:1-2<193 2015 404 11010 https://doi.org/10.1007/s11010-015-2378-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2378-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rosas-Díaz Marisol Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Camino de la Arboleda 300, Ex Hacienda de San José La Huerta., C.P. 58341, Morelia, Michoacán, Mexico aut NATIONALLICENCE 700 1- Camarillo-Cadena Menandro Department of Physical Chemistry, Univesidad Autónoma Metropolitana de Iztapalapa (UAM-Iztapalapa), Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Hernández-Arana Andrés Department of Physical Chemistry, Univesidad Autónoma Metropolitana de Iztapalapa (UAM-Iztapalapa), Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Ramón-Gallegos Eva Environmental Cytopathology Laboratory, ENCB-IPN, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Medina-Navarro Rafael Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Camino de la Arboleda 300, Ex Hacienda de San José La Huerta., C.P. 58341, Morelia, Michoacán, Mexico aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 193-201 0300-8177 404:1-2<193 2015 404 11010