caa a22 4500 606233717 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2371-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2371-9 Identification and bioinformatics analysis of miRNAs involved in bovine skeletal muscle satellite cell myogenic differentiation [Elektronische Daten] [Yi Wang, Xiang Ding, Yang Dai, Xin Liu, Hong Guo, Yong Zhang] MicroRNAs (miRNAs) are short non-coding RNA molecules that perform post-transcriptional repression of target genes by binding to 3′ untranslated regions, and involved in the regulation of many biological processes. Some studies indicate that miRNAs are mechanistically involved in the muscle growth and differentiation. However, little is known about miRNAs expression patterns during the process of bovine skeletal muscle satellite cell myogenic differentiated into myotubes. To investigate the mechanisms of miRNAs-mediated regulation during this process, we performed a miRNAs microarray to detect 783 bovine miRNAs in bovine skeletal muscle satellite cell myogenic differentiation, and the results were further confirmed by a quantitative real-time RT-PCR assay. We observed that the expression of 15 miRNAs was significantly different between bovine skeletal muscle satellite cells and differentiated myotubes, in which twelve were significantly up-regulated and three were down-regulated in myotubes. Furthermore, using bioinformatics methods, the targets of differentially expressed miRNAs were predicted, and were further subjected to gene ontology (GO) and KEGG analysis. A total of 3077 potential target genes were produced, and the highly enriched GOs and KEGG pathways showed that these genes together formed a regulatory network that involved in cell proliferation, cell differentiation, and multiple biological molecular signaling processes. Taken together, the results of the current study suggested the potential regulating roles of these differentially expressed miRNAs in bovine myogenic differentiation. Springer Science+Business Media New York, 2015 MicroRNAs nationallicence Bovine nationallicence Skeletal muscle satellite cells nationallicence Myogenic differentiation nationallicence Wang Yi Institute of Bioengineering, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Ding Xiang College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut Dai Yang College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut Liu Xin College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut Guo Hong College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut Zhang Yong Institute of Bioengineering, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 113-122 0300-8177 404:1-2<113 2015 404 11010 https://doi.org/10.1007/s11010-015-2371-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2371-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yi Institute of Bioengineering, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 700 1- Ding Xiang College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut NATIONALLICENCE 700 1- Dai Yang College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut NATIONALLICENCE 700 1- Liu Xin College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut NATIONALLICENCE 700 1- Guo Hong College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, 300384, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Yong Institute of Bioengineering, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 113-122 0300-8177 404:1-2<113 2015 404 11010