caa a22 4500 606233725 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2386-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2386-2 Protective effects of intestinal trefoil factor (ITF) on gastric mucosal epithelium through activation of extracellular signal-regulated kinase 1/2 (ERK1/2) [Elektronische Daten] [Jinfeng Lin, Zhaorui Sun, Wei Zhang, Hongmei Liu, Danbing Shao, Yi Ren, Yanfang Wen, Liping Cao, Joy Wolfram, Zhizhou Yang, Shinan Nie] The rapid repair of gastric mucosa is critical upon exposure to injurious agents. Intestinal trefoil factor (ITF) is a member of the trefoil factor family domain peptides, which play an important role in the cytoprotection of gastric epithelium. However, the underlying molecular mechanisms that are responsible for ITF-induced gastric epithelial repair remain unclear. In the present study, we demonstrate that ITF enhances the proliferation and migration of GES-1 gastric endothelial cells in a dose- and time-dependent manner through the activation of extracellular signal-regulated kinase 1/2 (ERK1/2). Furthermore, the ITF-mediated protection of GES-1 cells from a NS398 (nonsteroidal anti-inflammatory drug) was dependent on the ERK1/2 signaling pathway. Taken together, the results provide a mechanistic explanation for ITF-mediated protection of gastric epithelial mucosa cells, suggesting that activation of the ERK1/2 signaling pathway may provide a new therapeutic strategy for repairing gastric injury. Springer Science+Business Media New York, 2015 Intestinal trefoil factor nationallicence Gastric mucosal epithelium nationallicence Cytoprotection nationallicence ERK1/2 signaling pathway nationallicence Lin Jinfeng Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Sun Zhaorui Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Zhang Wei Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Liu Hongmei Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Shao Danbing Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Ren Yi Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Wen Yanfang Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Cao Liping Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Wolfram Joy National Center for Nanoscience & Technology of China, 100190, Beijing, People's Republic of China aut Yang Zhizhou Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Nie Shinan Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 263-270 0300-8177 404:1-2<263 2015 404 11010 https://doi.org/10.1007/s11010-015-2386-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2386-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Jinfeng Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Sun Zhaorui Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wei Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Hongmei Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Shao Danbing Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Ren Yi Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wen Yanfang Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Cao Liping Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wolfram Joy National Center for Nanoscience & Technology of China, 100190, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yang Zhizhou Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Nie Shinan Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, 210002, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 263-270 0300-8177 404:1-2<263 2015 404 11010