caa a22 4500 606233733 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2377-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2377-3 Alterations in gene promoter methylation and transcript expression induced by cisplatin in comparison to 5-Azacytidine in HeLa and SiHa cervical cancer cell lines [Elektronische Daten] [Swati Sood, Radhika Srinivasan] Despite recent advances in treatment, cervical cancer still remains one of the leading causes of cancer related mortality among women worldwide including India. Chemoradiation treatment is the standard-of-care which involves administration of cisplatin, a radiosensitizer along with radiation. The epigenetic changes induced by cisplatin are not known and so we designed this in vitro experimental study. We evaluated the changes induced by cisplatin administration in gene promoter methylation and the transcript levels of set of 7 genes and compared it to the changes induced by 5-Azacytidine, a known demethylating agent in two cervical cancer cell lines: HeLa (adenocarcinoma derived) and SiHa (squamous cell carcinoma derived) cell lines. Overall, there was a pronounced cytotoxic and growth inhibitory effect of both the drugs alone and in combination for both the cell lines which was dose and time dependent. Cisplatin as well as 5-Azacytidine treatment affected gene promoter methylation status resulting in demethylation and re-expression of the genes under investigation which was more pronounced in case of SiHa cells as compared to HeLa cells. Further, both the drugs acted in synergism as evident from their combination treatment. Therefore, at the cellular level, cisplatin and 5-Azacytidine can induce epigenetic changes in gene promoter methylation with altered expression which can have implications for treatment of cervical cancer. Springer Science+Business Media New York, 2015 Cisplatin nationallicence 5-Azacytidine nationallicence Methylation nationallicence Transcript expression nationallicence SiHa nationallicence HeLa nationallicence Sood Swati Molecular Pathology Laboratory, Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Research A- Block, Chandigarh, India aut Srinivasan Radhika Molecular Pathology Laboratory, Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Research A- Block, Chandigarh, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 181-191 0300-8177 404:1-2<181 2015 404 11010 https://doi.org/10.1007/s11010-015-2377-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2377-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sood Swati Molecular Pathology Laboratory, Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Research A- Block, Chandigarh, India aut NATIONALLICENCE 700 1- Srinivasan Radhika Molecular Pathology Laboratory, Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Research A- Block, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 181-191 0300-8177 404:1-2<181 2015 404 11010