caa a22 4500 60623375X CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2365-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2365-7 Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation [Elektronische Daten] [Veronika Gurianova, Dmytro Stroy, Peter Kruzliak, Victoria Kyrichenko, Alex Moibenko, Victor Dosenko] Proteasome and microRNAs play a critical role in almost all processes in a living organism, including pathology of the heart; however, their interaction is still in question. In the present study, we have found that proteasome inhibitor provoked increase of mature but not immature microRNA-1 in cultured cardiomyocytes, and tested the hypothesis that mature microRNA-1 can be a substrate for endonuclease activity of proteasome. In our in vitro experiments, we have found that proteasome fraction II is able to degrade both mature and primary but not precursor microRNA-1. However, this in vitro effect was not abolished by chemical inhibitor of proteolytic activities of proteasome. These data let us summarize that proteasome has the complex effect on the level of microRNA-1. Springer Science+Business Media New York, 2015 MicroRNA nationallicence Proteasome nationallicence Cardiomyocytes nationallicence Anoxia-reoxygenation nationallicence Gurianova Veronika Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut Stroy Dmytro Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut Kruzliak Peter Department of Cardiovascular Diseases, International Clinical Research Center, St. Anne´s University Hospital, Masaryk University, Pekarska 53, 656 91, Brno, Czech Republic aut Kyrichenko Victoria Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut Moibenko Alex Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut Dosenko Victor Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 45-51 0300-8177 404:1-2<45 2015 404 11010 https://doi.org/10.1007/s11010-015-2365-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2365-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gurianova Veronika Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut NATIONALLICENCE 700 1- Stroy Dmytro Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut NATIONALLICENCE 700 1- Kruzliak Peter Department of Cardiovascular Diseases, International Clinical Research Center, St. Anne´s University Hospital, Masaryk University, Pekarska 53, 656 91, Brno, Czech Republic aut NATIONALLICENCE 700 1- Kyrichenko Victoria Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut NATIONALLICENCE 700 1- Moibenko Alex Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut NATIONALLICENCE 700 1- Dosenko Victor Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 45-51 0300-8177 404:1-2<45 2015 404 11010