caa a22 4500 606233792 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2379-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2379-1 Clopidogrel reduces apoptosis and promotes proliferation of human vascular endothelial cells induced by palmitic acid via suppression of the long non-coding RNA HIF1A-AS1 in vitro [Elektronische Daten] [Jing Wang, Lingqiang Chen, Hongfei Li, Jin Yang, Zhiqiang Gong, Bing Wang, Xueling Zhao] Cardiovascular disease (CVD) is recognized as a major and increasing health problem affected older subjects in China, and clopidogrel has been widely used for treatment of CVD patients such as atherosclerosis, myocardial infarction, and myocardial ischaemia-reperfusion damage. However, the molecular mechanisms of clopidogrel for treatment of CVD are only partially understood. This study investigated the effects of clopidogrel on palmitic acid-induced damage of human vascular endothelial cells (HUVECs), and the molecular mechanisms of LncRNA HIF1A-AS1 in regulating the proliferation and apoptosis of HUVECs in vitro. We firstly established a damage model of HUVECs through palmitic acid (PA) treatment. And the effect of clopidogrel reducing PA-induced apoptosis of HUVECs was observed by the flow cytometric measurement. To further understand the molecular mechanism of clopidogrel rescues PA-induced apoptosis, we used human LncRNA PCR array to compare the LncRNA expression profile difference between clopidogrel-treated cells and control cells. The expression of LncRNA HIF 1 alpha-antisense RNA 1 (HIF1A-AS1) was significantly altered in clopidogrel-treated cells. We further proved that suppression of HIF1A-AS1 by siRNA reduce PA-induced apoptosis and promote proliferation of HUVECs. Furthermore, we also demonstrated inhibition apoptosis effect by HIF1A-AS1 is related to mitochondrial apoptosis pathway. Hence, our results suggest that clopidogrel rescues apoptosis and promotes proliferation of PA-induced damage model of HUVECs through inhibiting the mediator LncRNA HIF1A-AS1. These findings indicate that LncRNA HIF1A-AS1 may play an important role in the pathogenesis of CVD, and provide a novel molecular mechanism of clopidogrel for treatment of CVD. Springer Science+Business Media New York, 2015 Human vascular endothelial cells nationallicence Palmitic acid nationallicence Apoptosis nationallicence Clopidogrel nationallicence Long non-coding RNA HIF1A-AS1 nationallicence Bcl 2-caspase 9-caspase 3 pathway nationallicence Wang Jing Department of Rheumatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China aut Chen Lingqiang Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Li Hongfei Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Yang Jin Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Gong Zhiqiang Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Wang Bing Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Zhao Xueling Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 203-210 0300-8177 404:1-2<203 2015 404 11010 https://doi.org/10.1007/s11010-015-2379-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2379-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jing Department of Rheumatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Chen Lingqiang Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Li Hongfei Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Yang Jin Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Gong Zhiqiang Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Wang Bing Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Zhao Xueling Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, 650032, Kunming, Yunnan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 203-210 0300-8177 404:1-2<203 2015 404 11010