caa a22 4500 606233822 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-015-2326-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2326-1 Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats [Elektronische Daten] [Luiz de Andrade, Wilson de Moraes, Eduardo Matsuo Junior, Elizabeth de Orleans Carvalho de Moura, Hanna Antunes, Jairo Montemor, Ednei Antonio, Danilo Bocalini, Andrey Serra, Paulo Tucci, Patricia Brum, Alessandra Medeiros] The activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. The aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wistar and SHR rats were randomly divided into sedentary and trained groups. The AET protocol was 5×/week in treadmill for 13weeks. Exercise tolerance test, non-invasive blood pressure measurement, echocardiographic analyses, and left ventricle hemodynamics were performed during experimental period. The expression of ubiquitinated proteins, 4-hydroxynonenal (4-HNE), Akt, phospho-Aktser473, GSK3β, and phospho-GSK3βser9 were analyzed by western blotting. The evaluation of lipid hydroperoxide concentration was performed using the xylenol orange method, and the proteasomal chymotrypsin-like activity was measured by fluorimetric assay. Sedentary hypertensive group presented cardiac hypertrophy, unaltered expression of total Akt, phospho-Akt, total GSK3β and phospho-GSK3β, UPS hyperactivity, increased lipid hydroperoxidation as well as elevated expression of 4-HNE but normal cardiac function. In contrast, AET significantly increased exercise tolerance, decreased resting systolic blood pressure and heart rate in hypertensive animals. In addition, the AET increased phospho-Akt expression, decreased phospho-GSK3β, and did not alter the expression of total Akt, total GSK3β, and ubiquitinated proteins, however, significantly attenuated 4-HNE levels, lipid hydroperoxidation, and UPS's activity toward normotensive group levels. Our results provide evidence for the main effect of AET on attenuating cardiac ubiquitin proteasome hyperactivity and oxidative stress in SHR rats. Springer Science+Business Media New York, 2015 Hypertension nationallicence Aerobic exercise training nationallicence Cardiac remodeling nationallicence Ubiquitin proteasome system nationallicence Oxidative stress nationallicence de Andrade Luiz Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut de Moraes Wilson Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut Matsuo Junior Eduardo Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut de Orleans Carvalho de Moura Elizabeth Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut Antunes Hanna Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut Montemor Jairo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut Antonio Ednei Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut Bocalini Danilo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut Serra Andrey Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut Tucci Paulo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut Brum Patricia School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil aut Medeiros Alessandra Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 193-202 0300-8177 402:1-2<193 2015 402 11010 https://doi.org/10.1007/s11010-015-2326-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2326-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Andrade Luiz Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 700 1- de Moraes Wilson Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 700 1- Matsuo Junior Eduardo Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 700 1- de Orleans Carvalho de Moura Elizabeth Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 700 1- Antunes Hanna Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 700 1- Montemor Jairo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Antonio Ednei Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Bocalini Danilo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Serra Andrey Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Tucci Paulo Cardio-Physiology and Pathophysiology Laboratory, Federal University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Brum Patricia School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Medeiros Alessandra Universidade Federal de São Paulo- Departamento de Biociências, Silva Jardim, 136-Vl. Mathias, 11015-020, Santos, SP, Brazil aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 193-202 0300-8177 402:1-2<193 2015 402 11010