caa a22 4500 606233849 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-015-2331-4 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2331-4 Maclurin suppresses migration and invasion of human non-small-cell lung cancer cells via anti-oxidative activity and inhibition of the Src/FAK-ERK-β-catenin pathway [Elektronische Daten] [Min Ku, Ji Kim, Jongsung Lee, Jae Cho, Taehoon Chun, Sang Lee] Recent reports indicated that ROS is closely related with cancer metastasis. ROS targets major signaling molecules which are known to be involved in migration and invasion of cancer cells. Here we report that maclurin, a major phenolic component of ethanol extracted mulberry twigs, exerts anti-metastatic effect in A549 human non-small-cell lung cancer cells. Maclurin suppresses intracellular ROS level in A549 human non-small-cell lung cancer cells. Also, maclurin down-regulates Src and ERK, which are well known to be regulated with redox state. Suppressed Src/FAK and ERK signalings activate GSK3-β, thus inhibiting nuclear accumulation of β-catenin. As a result, transcriptional expressions of two major gelatinases (MMP-2 and MMP-9) were significantly down-regulated. Consequently, migration and invasion of A549 human non-small-cell lung cancer cells were attenuated. Anti-metastatic effect of maclurin on A549 human non-small-cell lung cancer cells were diminished by the treatment of hydrogen peroxide, thus further implicating that the effect of maclurin may be strongly related with its anti-oxidative activity. Thus, our data indicate that the anti-metastatic effect of maclurin is exerted by anti-oxidative activity and inhibition of Src/FAK-ERK-β-catenin signaling pathway. Springer Science+Business Media New York, 2015 Non-small-cell lung cancer nationallicence Reactive oxygen species nationallicence Maclurin nationallicence Metastasis nationallicence Matrix metalloproteinases nationallicence NSCLC : Non-small-cell lung cancer nationallicence MMP : Matrix metalloproteinase nationallicence MAPK : Mitogen-activated protein kinase nationallicence ROS : Reactive oxygen species nationallicence Ku Min Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut Kim Ji Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut Lee Jongsung Department of Dermatological Health Management, College of Health Science, Eulji University, 561-713, Seongnam-Si, Gyeonggi-Do, Korea aut Cho Jae Department of Genetic Engineering, Sungkyunkwan University, 440-746, Suwon-Si, Gyeonggi-Do, Korea aut Chun Taehoon Division of Biotechnology, Korea University, 136-701, Seoul, Korea aut Lee Sang Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 243-252 0300-8177 402:1-2<243 2015 402 11010 https://doi.org/10.1007/s11010-015-2331-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2331-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ku Min Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Kim Ji Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Lee Jongsung Department of Dermatological Health Management, College of Health Science, Eulji University, 561-713, Seongnam-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Cho Jae Department of Genetic Engineering, Sungkyunkwan University, 440-746, Suwon-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 700 1- Chun Taehoon Division of Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Sang Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, 461-701, Seongnam-Si, Gyeonggi-Do, Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 243-252 0300-8177 402:1-2<243 2015 402 11010