caa a22 4500 606233865 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2295-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2295-9 Differential expression and DNA methylation of angiotensin type 1A receptorsin vascular tissues during genetic hypertension development [Elektronische Daten] [Fang Pei, Xinquan Wang, Rongchuan Yue, Caiyu Chen, Ji Huang, Jie Huang, Xiaohui Li, Chunyu Zeng] Angiotensin type 1a receptor (AT1aR) is thought to play an important role in the development of hypertension. However, it is unknown how the AT1aR expression in vascular tissue is changed during the development of hypertension or if the degree of methylation in the AT1aR promoter correlates with the expression of AT1aR. To address these questions, we measured AT1aR mRNA, protein expression, and methylation status of the AT1aR promoter in the aorta and mesenteric artery of male spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats acting as controls at pre-hypertensive (4weeks), evolving (10weeks), and established (20weeks) stages of hypertension. The expression of the AT1aR mRNA and protein was not different between the SHRs and WKY rats at 4weeks. However, they were significantly greater in SHRs than in WKY rats at 20weeks. Bisulfite sequencing revealed that the AT1aR promoter from the aorta and mesenteric artery of the SHRs was progressively hypo-methylated with age as compared with their WKY rat counterparts. These results suggest that the heightened AT1aR expression in SHRs is related to the AT1aR promoter hypo-methylation, which might be a consequence of the increased blood pressure and may be important in the maintenance of high blood pressure. Springer Science+Business Media New York, 2015 Hypertension nationallicence AT1 receptor nationallicence DNA methylation nationallicence Artery nationallicence SHRs nationallicence Pei Fang Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, 30 Gaotanyan, Shapingba District, 400038, Chongqing, China aut Wang Xinquan Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut Yue Rongchuan Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut Chen Caiyu Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut Huang Ji Department of Cardiology, Chongqing Corps Hospital of Chinese People's Armed Police Force, No. 90, Marbles Shi Weiguo Road, Nanan District, 400061, Chongqing, China aut Huang Jie Department of Cardiology, Chongqing Corps Hospital of Chinese People's Armed Police Force, No. 90, Marbles Shi Weiguo Road, Nanan District, 400061, Chongqing, China aut Li Xiaohui Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, 30 Gaotanyan, Shapingba District, 400038, Chongqing, China aut Zeng Chunyu Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 1-8 0300-8177 402:1-2<1 2015 402 11010 https://doi.org/10.1007/s11010-014-2295-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2295-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pei Fang Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, 30 Gaotanyan, Shapingba District, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Wang Xinquan Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Yue Rongchuan Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Chen Caiyu Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Huang Ji Department of Cardiology, Chongqing Corps Hospital of Chinese People's Armed Police Force, No. 90, Marbles Shi Weiguo Road, Nanan District, 400061, Chongqing, China aut NATIONALLICENCE 700 1- Huang Jie Department of Cardiology, Chongqing Corps Hospital of Chinese People's Armed Police Force, No. 90, Marbles Shi Weiguo Road, Nanan District, 400061, Chongqing, China aut NATIONALLICENCE 700 1- Li Xiaohui Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, 30 Gaotanyan, Shapingba District, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Zeng Chunyu Department of Cardiology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Rd, Yuzhong District, 400042, Chongqing, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 1-8 0300-8177 402:1-2<1 2015 402 11010