caa a22 4500 606233873 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2321-y doi (NATIONALLICENCE)springer-10.1007/s11010-014-2321-y Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines, up-regulation of Bcl-2, down-regulation of Bax, and caspase-3 [Elektronische Daten] [Lavanya Yaidikar, Santhrani Thakur] Punicalagin (PG) is a hydrolysable tannin compound found in Punica granatum L. The purpose of the present work is to explore the neuroprotective mechanism of PG against ischemia-reperfusion (I/R) injury in rat model of middle cerebral artery occlusion (MCAO). Rats were randomly divided into sham, MCAO, and PG-treated groups. PG (15 and 30mg/kg), the vehicle was administered orally for 7days prior to MCAO. Rats were anesthetised with ketamine (100mg/kg/im), xylazine (10mg/kg/im) and subjected to 2h occlusion and 22h reperfusion. The effects of PG on behavioral deficit and infarct volume, the levels of glutamate and calcium as well as the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) were evaluated. Moreover, the expressions of caspase-3, Bcl-2, and Bax were detected by Western blotting. As compared with MCAO group, PG-treated rats showed dose-dependent reduction in infarct volume and substantial improvement in behavioral deficit. The levels of glutamate, calcium, TNF-α, IL-1β, and IL-6 were restored significantly. The Western blotting results revealed that the expression of Bcl-2 was up-regulated and that of caspase-3, Bax were down-regulated when exposed to PG. From our results, it can be concluded that PG showed an ameliorative effect against cerebral I/R injury in rats through its anti-inflammatory, antioxidant actions besides it inhibits excitotoxicity. It also suppresses apoptosis through regulating, Bcl-2, caspase-3, and Bax protein expressions, perhaps another mechanism by which PG employs its neuroprotective action. Springer Science+Business Media New York, 2015 Cerebral ischemia-reperfusion injury nationallicence Punicalagin nationallicence Neuroprotection nationallicence Anti-inflammatory action nationallicence Anti-apoptotic activity nationallicence Yaidikar Lavanya Division of Pharmacology Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), 517502, Tirupati, Andhra Pradesh, India aut Thakur Santhrani Division of Pharmacology Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), 517502, Tirupati, Andhra Pradesh, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 141-148 0300-8177 402:1-2<141 2015 402 11010 https://doi.org/10.1007/s11010-014-2321-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2321-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yaidikar Lavanya Division of Pharmacology Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), 517502, Tirupati, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Thakur Santhrani Division of Pharmacology Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), 517502, Tirupati, Andhra Pradesh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 141-148 0300-8177 402:1-2<141 2015 402 11010