caa a22 4500 60623392X CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2318-6 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2318-6 Calcitonin gene-related peptide stimulates proliferation and osteogenic differentiation of osteoporotic rat-derived bone mesenchymal stem cells [Elektronische Daten] [Wei Liang, Xianglong Zhuo, Zhongfei Tang, Xiaomou Wei, Bing Li] Osteoporosis, a systemic bone disorder, is prevalent in postmenopausal woman. Bone mesenchymal stem cells (BMSCs), precursors of osteogenic cells, may contribute to prevention or treatment of bone frustrate in osteoporosis. Recently, two studies suggested a role of calcitonin gene-related peptide (CGRP) in promoting osteogenesis of BMSCs under physiological conditions. However, the role of CGRP on BMSCs, which are derived from osteoporotic tissues, is unclear. Here, we investigated the role of CGRP on BMSCs isolated from female osteoporotic rats. Data showed that CGRP stimulated cell proliferation and inhibited cell apoptosis for short-term culture of BMSCs. Instead, CGRP induced BMSCs differentiation into the osteoblasts and promoted formation of calcified nodules after long-term culture. Moreover, CGRP gradually up-regulated expression levels of osteoporotic differentiation-related genes including alkaline phosphatase, Collagen type I, Bmp2, Osteonectin, and Runx2 during osteogenic differentiation. In conclusion, CGRP promoted proliferation and induced osteogenic differentiation and mineralization during female osteoporotic rat-derived BMSC differentiation. These findings support a potential role of CGRP on the prevention or treatment of osteoporotic fracture. Springer Science+Business Media New York, 2015 Calcitonin gene-related peptide nationallicence Osteoporosis nationallicence Bone mesenchymal stem cells nationallicence Osteogenic differentiation nationallicence Liang Wei The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut Zhuo Xianglong The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut Tang Zhongfei The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut Wei Xiaomou The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut Li Bing The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 101-110 0300-8177 402:1-2<101 2015 402 11010 https://doi.org/10.1007/s11010-014-2318-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2318-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liang Wei The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut NATIONALLICENCE 700 1- Zhuo Xianglong The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut NATIONALLICENCE 700 1- Tang Zhongfei The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut NATIONALLICENCE 700 1- Wei Xiaomou The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut NATIONALLICENCE 700 1- Li Bing The Fourth Affiliated Hospital, Guangxi Medical University, No. 1 Liushi Road, Yufeng, 545005, Liuzhou, Guangxi, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 101-110 0300-8177 402:1-2<101 2015 402 11010