caa a22 4500 606233938 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2315-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2315-9 Increased expression of SOX4 is a biomarker for malignant status and poor prognosis in patients with non-small cell lung cancer [Elektronische Daten] [Dingmiao Wang, Ting Hao, Yang Pan, Xiaowei Qian, Daixing Zhou] The aim of the present study was to analyze the expression of sex-determining region Y-related high mobility group box 4 (SOX4) in non-small cell lung cancer (NSCLC) and its correlation with clinicopathologic characteristics, including the survival of NSCLC patients. To observe initially the expression status of SOX4 in lung squamous cell carcinoma and adenocarcinoma at gene expression omnibus. The expression of SOX4 mRNA and protein was examined in NSCLC tissues and normal lung tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of SOX4 expression levels with clinical characteristics of 168 NSCLC patients was analyzed by immunohistochemistry. Univariate and multivariate analyses were performed to determine the association between SOX4 expression and prognosis of NSCLC patients. In our results, SOX4 expression was increased in NSCLC tissues compared with paired normal lung tissues in microarray data (GSE3268). SOX4 mRNA and protein expression were markedly higher in NSCLC tissues than in normal lung tissues (P=0.001 and P=0.001, respectively). Using immunohistochemistry, high levels of SOX4 protein were positively correlated with status of differentiated degree (high vs. middle, P=0.004; high vs. low, P<0.001), clinical stage (I-II vs. III-IV, P<0.001), T classification (T1-T2 vs. T3-T4, P=0.004), N classification (N0-N1 vs. N2-N3, P=0.002), and M classification (M0 vs. M1, P=0.011) in NSCLC. Moreover, the higher level of SOX4 expression was markedly correlated with poor overall survival in NSCLC patients (P<0.001). Multivariate analysis suggested that increased SOX4 expression was a poor independent prognostic predictor for NSCLC patients (P=0.002). In conclusion, SOX4 plays an important role on NSCLC progression and prognosis and may serve as a convictive prognostic biomarker for NSCLC patients. Springer Science+Business Media New York, 2015 SOX4 nationallicence Non-small cell lung cancer nationallicence Biomarker nationallicence Prognosis nationallicence Wang Dingmiao Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut Hao Ting Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut Pan Yang Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut Qian Xiaowei Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut Zhou Daixing Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Road, 430030, Wuhan, Hubei, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 75-82 0300-8177 402:1-2<75 2015 402 11010 https://doi.org/10.1007/s11010-014-2315-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2315-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Dingmiao Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut NATIONALLICENCE 700 1- Hao Ting Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut NATIONALLICENCE 700 1- Pan Yang Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut NATIONALLICENCE 700 1- Qian Xiaowei Department of Emergency Medicine, Xianning Central Hospital, 437100, Xianning, Hubei, China aut NATIONALLICENCE 700 1- Zhou Daixing Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Road, 430030, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 75-82 0300-8177 402:1-2<75 2015 402 11010