caa a22 4500 606233954 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2319-5 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2319-5 MiR-214 regulates the pathogenesis of patients with coronary artery disease by targeting VEGF [Elektronische Daten] [Yan Jin, Cheng-Jian Yang, Xin Xu, Jia-Ning Cao, Qiu-Ting Feng, Jun Yang] Circulating microRNAs (miRNAs) in patient body fluids have recently been considered to hold the potential of being novel disease biomarkers and drug targets. We aimed to investigate the correlation between the levels of circulating miR-214 and the expression of vascular endothelial growth factor (VEGF) in the pathogenesis of coronary heart disease patients to further explore the mechanism involved in the vasculogenesis. Three different cohorts, including 13 acute myocardial infarction patients, 176 angina pectoris patients, and 127 control subjects, were enrolled to investigate the expression levels of circulating miR-214 in patients with myocardial ischemia and also the relationship between plasma miR-214 and severity of coronary stenosis. Plasma miR-214 levels of participants were examined by real-time quantitative PCR. Simultaneously, plasma cardiac troponin I concentrations were measured by ELISA assays. We further detected the correlation of miR-214 and VEGF by molecular and animal assays. MiR-214 was enriched in not only diseased endothelial progenitor cells (EPCs) but also the plasma of coronary artery disease (CAD) patients. Besides, we found out miR-214 was able to suppress VEGF expression and EPC activities. Reporter assays confirmed the direct binding and repression of miR-214 to the 39-UTR of VEGF mRNA. Knockdown of miR-214 not only restored VEGF levels and angiogenic activities of diseased EPCs in vitro, but also further promoted blood flow recovery in ischemic limbs of mice. Circulating miR-214 may be a new biomarker for CAD and as a potential diagnostic tool. And increased miR-214 level may be used to predict the presence and severity of coronary lesions in CAD patients. Springer Science+Business Media New York, 2015 MiR-214 nationallicence VEGF nationallicence Endothelial progenitor cells (EPCs) nationallicence Coronary artery disease (CAD) nationallicence Disease marker nationallicence Jin Yan Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Yang Cheng-Jian Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Xu Xin Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Cao Jia-Ning Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Feng Qiu-Ting Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Yang Jun Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 111-122 0300-8177 402:1-2<111 2015 402 11010 https://doi.org/10.1007/s11010-014-2319-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2319-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Yan Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Yang Cheng-Jian Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Xu Xin Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Cao Jia-Ning Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Feng Qiu-Ting Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Yang Jun Department of Cardiology, Wuxi No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, 214002, Wuxi, Jiangsu, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 111-122 0300-8177 402:1-2<111 2015 402 11010