caa a22 4500 606233962 CHVBK 20210128101234.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2320-z doi (NATIONALLICENCE)springer-10.1007/s11010-014-2320-z Limbal epithelial stem-microenvironmental alteration leads to pterygium development [Elektronische Daten] [Prosun Das, Arjun Gokani, Ketaki Bagchi, Gautam Bhaduri, Samaresh Chaudhuri, Sujata Law] Maintenance of tissue homeostasis relies on the accurate regulation of tissue specific stem cell activity which is governed by the dynamic interaction between the positive and negative feedback modulating mechanism of stem cell microenvironmental niche. Alteration or deregulation of the "stem-microenvironmental networking” provokes disease development. Limbal epithelial stem cells (LESC) are the initiator hierarchy that maintains corneal integrity. Compartmentalization of LESC within the limbal vicinity provides an opportunity to understand the stem-microenvironmental relationship. The purpose of this study was to determine the microenvironmental alteration associated with LESCs fate in pterygium condition in comparison with healthy state. Clinical observations evaluated the ocular surface disorder with respect to corneal vascularization, tear film abnormality, and thickening of limbal area in pterygium patients. Structural alteration of limbal stem/progenitor cells and its neighboring niche components were observed using histology and scanning electron microscopy. Receptor overexpression of TGFβ-R1, EGF-R1, and IL6-Rα and alteration of IL2-Rα expression pointed toward aberration of "stem-microenvironmental networking” in the limbal vicinity during disease development. Increased cell proliferation index along with TERT, Cyclin-D1, and PCNA over-expression in limbal part of pterygium epithelial cells indicated increased cellular proliferation and disturbed homeostatic equilibrium. We postulate that pterygium is associated with limbal microenvironmental anomaly where the resident epithelial cells became hyperproliferative. Springer Science+Business Media New York, 2015 Pterygium nationallicence Stem-microenvironmental networks nationallicence Cytology nationallicence Scanning electron microscopy nationallicence Explant culture nationallicence Growth factor nationallicence Cell cycle nationallicence LESC : Limbal epithelial stem cells nationallicence UV-B : Ultra violet-B nationallicence TGFβ-R1 : Transforming growth factor beta-receptor 1 nationallicence EGF-R1 : Epidermal growth factor-receptor 1 nationallicence IL6-Rα : Interleukin 6- Receptor alpha nationallicence TERT : Telomerase reverse transcriptase nationallicence PCNA : Proliferating cell nuclear antigen nationallicence AS-OCT : Anterior segment optical coherence tomography nationallicence PCC : Peripheral corneal thickness nationallicence CCT : Central corneal thickness nationallicence SEM : Scanning electron microscopy nationallicence PI : Proliferation index nationallicence LESCDD : Limbal stem cell deficiency diseases nationallicence EMT : Epithelial-mesenchymal transition nationallicence MAPK : Mitogen activated protein kinase nationallicence gp130 : Glycoprotein 130 nationallicence FBS : Fetal bovine serum nationallicence Das Prosun Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Gokani Arjun Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Bagchi Ketaki Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Bhaduri Gautam Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Chaudhuri Samaresh Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Law Sujata Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 123-139 0300-8177 402:1-2<123 2015 402 11010 https://doi.org/10.1007/s11010-014-2320-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2320-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Das Prosun Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 700 1- Gokani Arjun Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 700 1- Bagchi Ketaki Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 700 1- Bhaduri Gautam Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 700 1- Chaudhuri Samaresh Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 700 1- Law Sujata Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C R Avenue, 700073, Kolkata, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 123-139 0300-8177 402:1-2<123 2015 402 11010