caa a22 4500 606234004 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-015-2328-z doi (NATIONALLICENCE)springer-10.1007/s11010-015-2328-z Caveolin-1 mediates gene transfer and cytotoxicity of polyethyleneimine in mammalian cell lines [Elektronische Daten] [Hai-Jie Yang, Pei Feng, Lei Wang, Zhi-Chao Li, Shuang-Ping Ma, Mian Wang, Zhi-Wei Feng] Polyethyleneimine (PEI) is a cost-effective and non-viral vector for gene transfer,but the factors determining gene transfer efficiency and cytotoxicity of PEI in different mammalian cell lines remain largely unknown. In the present study, three different cell lines were chosen for investigation. Using pEGFP DNA and PEI, 21.5, 29.2, and 92.1% of GFP-positive cells were obtained in BMSC, Hela, and 293T, respectively. In luciferase reporter assay, similar results were obtained (for luciferase activity, BMSC<Hela<293T cells). By MTT test and cell apoptotic marker analysis, we demonstrated that high gene transfer efficiency is accompanied with high cytotoxicity of PEI. Moreover, we found that high expression level of caveolin-1 was accompanied with high gene transfer efficiency and cytotoxicity of PEI in 293T cells. More convincingly, caveolin-1 silencing in 293T could reduce both gene transfer efficiency and cytotoxicity of PEI. In contrast, caveolin-1 overexpression in BMSCs increases both gene transfer efficiency and cytotoxicity of PEI. Taken together, our study suggests that caveolin-1 may at least in part determine gene transfer efficiency and cytotoxicity of PEI in mammalian cell lines, providing caveolin-1 as a potential target for improving gene transfer efficiency when applying positively charged polyplexes to cell transfection. Springer Science+Business Media New York, 2015 Polyethyleneimine nationallicence Gene transfer nationallicence Cytotoxicity nationallicence Caveolin-1 nationallicence Gene silencing nationallicence Overexpression nationallicence Yang Hai-Jie College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Feng Pei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Wang Lei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Li Zhi-Chao College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Ma Shuang-Ping College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Wang Mian College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Feng Zhi-Wei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 203-211 0300-8177 402:1-2<203 2015 402 11010 https://doi.org/10.1007/s11010-015-2328-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2328-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Hai-Jie College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Feng Pei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Wang Lei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Li Zhi-Chao College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Ma Shuang-Ping College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Wang Mian College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Feng Zhi-Wei College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 203-211 0300-8177 402:1-2<203 2015 402 11010