caa a22 4500 606234020 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11010-014-2317-7 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2317-7 Quercetin inhibits proliferation and invasion acts by up-regulating miR-146a in human breast cancer cells [Elektronische Daten] [Si-feng Tao, Hai-fei He, Qiang Chen] Breast cancer is the most common female malignancies in the world which seriously impacts the female health. In recent years, various studies have been reported to determine the relevance of miRNAs to human cancer. One of these miRNAs, miR-146a has been down-regulated in multiple human cancer types, but up-regulation showed inducing apoptosis. To determine the role of quercetin treated on breast cancer, we investigated the effect of quercetin on cell proliferation in human breast cancer cell lines MCF-7 and MDA-MB-231 with/without transfection of miR-146a mimic or anti-miR-146a. Furthermore, the expressions of bax and cleaved-caspase-3, mainly were increased in control and overexpression miR-146a groups, however, the expression of EGFR was inverse. All the results demonstrated that quercetin exhibited excellent effect on inhibiting cell proliferation in human breast cancer cells, which was performed by up-regulating miR-146a expression, then via inducing apoptosis through caspase-3 activation and mitochondrial-dependent pathways, and inhibiting invasion through down-regulating the expression of EGFR. Springer Science+Business Media New York, 2015 Quercetin nationallicence miRNAs nationallicence miR-146a nationallicence EGFR nationallicence Breast cancer nationallicence Tao Si-feng Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut He Hai-fei Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut Chen Qiang Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 93-100 0300-8177 402:1-2<93 2015 402 11010 https://doi.org/10.1007/s11010-014-2317-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2317-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tao Si-feng Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut NATIONALLICENCE 700 1- He Hai-fei Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut NATIONALLICENCE 700 1- Chen Qiang Department of Surgical Oncology, School of Medicine, Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 402/1-2(2015-04-01), 93-100 0300-8177 402:1-2<93 2015 402 11010