caa a22 4500 606234063 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2230-0 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2230-0 Molecular adaptations in vasoactive systems during acute stroke in salt-induced hypertension [Elektronische Daten] [Nicole Ventura, Nichole Peterson, M. Tse, R. Andrew, Stephen Pang, Albert Jin] Investigations regarding hypertension and dietary sodium, both factors that influence stroke risk, have previously been limited to using genetically disparate treatment and control groups, namely the stroke-prone, spontaneously hypertensive rat and Wistar-Kyoto rat. In this investigation, we have characterized and compared cerebral vasoactive system adaptations following stroke in genetically identical, salt-induced hypertensive, and normotensive control mice. Briefly, ANP+/− (C57BJ/6 × SV129 background) mice were fed chow containing either 0.8% NaCl (NS) or 8.0% NaCl (HS) for 7weeks. Transient cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). Infarct volumes were measured 24-h post-reperfusion and the mRNA expression of five major vasoactive systems was characterized using qPCR. Along with previous publications, our data validate a salt-induced hypertensive state in ANP+/− mice fed HS chow as they displayed left ventricular hypertrophy, increased systolic blood pressure, and increased urinary sodium excretion. Following MCAO, mice fed HS exhibited larger infarct volumes than their dietary counterparts. In addition, significant up-regulation in Et-1 and Nos3 mRNA expression in response to salt and stroke suggests implications with increased cerebral damage in this group. In conclusion, our data demonstrate increased cerebral susceptibility to stroke in salt-induced hypertensive mice. More importantly, however, we have characterized a novel method of investigating hypertension and stroke with the use of genetically identical treatment and control groups. This is the first investigation in which genetic confounding variables have been eliminated. Springer Science+Business Media New York, 2014 Stroke nationallicence Hypertension nationallicence Salt-sensitivity nationallicence Dietary sodium nationallicence Natriuretic peptide nationallicence Ventura Nicole Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut Peterson Nichole Department of Medicine (Neurology), Kingston General Hospital, 76 Stuart St., K7L 2V7, Kingston, ON, Canada aut Tse M. Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut Andrew R. Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut Pang Stephen Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut Jin Albert Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 39-47 0300-8177 399:1-2<39 2015 399 11010 https://doi.org/10.1007/s11010-014-2230-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2230-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ventura Nicole Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Peterson Nichole Department of Medicine (Neurology), Kingston General Hospital, 76 Stuart St., K7L 2V7, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Tse M. Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Andrew R. Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Pang Stephen Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Jin Albert Department of Biomedical and Molecular Sciences, Queen's University, Rm 850 Botterell Hall 18 Stuart St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 39-47 0300-8177 399:1-2<39 2015 399 11010