caa a22 4500 60623408X CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2255-4 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2255-4 Caveolin-1 regulates metastatic behaviors of anoikis resistant lung cancer cells [Elektronische Daten] [Pithi Chanvorachote, Varisa Pongrakhananon, Hasseri Halim] Caveolin-1 (Cav-1), a protein component of cellular membrane, has been reported to regulate several cancer cell behaviors. However, its role on cancer metastasis in anoikis resistant cells is unknown. The present study aimed to investigate the correlation between Cav-1 level and aggressive behaviors of anoikis resistant cancer cells. Cav-1 and ShRNACav-1 stably transfected lung carcinoma cells, and anoikis resistant H_AR1 and H_AR2 cells expressing different levels of Cav-1 were subjected to anoikis, cell growth, anchorage-independent growth, extracellular matrix adhesion, cisplatin sensitivity, migration, and invasion assays. The correlations between cellular Cav-1 level and such cancer aggressive behaviors were evaluated. Results revealed that anoikis resistant lung cancer cells as well as Cav-1 overexpressing cells exhibit a significant increase in anchorage-independent growth, extracellular matrix adhesion, migration, and invasion in comparison to those of their parental H460 cells. Knock-down of Cav-1 by ShRNA transfection was able to reverse such metastatic potentials in H_AR2 cells. In addition, basal Cav-1 level of these cells was positively correlated with anoikis resistance, anchorage-independent growth, migration, and invasion behaviors of the cells, whereas such Cav-1 level showed poor correlation to cisplatin sensitivity, cell adhesion, and growth in attached condition. These findings give more information regarding role of Cav-1 in the regulation of behaviors of lung cancer cells. Springer Science+Business Media New York, 2014 Lung cancer nationallicence Anoikis resistance nationallicence Caveolin-1 nationallicence Cell aggressiveness nationallicence Correlation nationallicence Cav-1 : Caveolin-1 nationallicence XTT : 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt nationallicence PBS : Phosphate-buffered saline nationallicence poly-HEMA : poly-2-hydroxyethyl methacrylate nationallicence ShRNA : Short hairpin RNA nationallicence TBST : Tris-buffered saline with 0.1% tween nationallicence PI : Propidium iodide nationallicence Chanvorachote Pithi Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, 10330, Bangkok, Thailand aut Pongrakhananon Varisa Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, 10330, Bangkok, Thailand aut Halim Hasseri Department of Pharmacology, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300, Bandar Puncak Alam, Selangor, Malaysia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 291-302 0300-8177 399:1-2<291 2015 399 11010 https://doi.org/10.1007/s11010-014-2255-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2255-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chanvorachote Pithi Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, 10330, Bangkok, Thailand aut NATIONALLICENCE 700 1- Pongrakhananon Varisa Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, 10330, Bangkok, Thailand aut NATIONALLICENCE 700 1- Halim Hasseri Department of Pharmacology, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300, Bandar Puncak Alam, Selangor, Malaysia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 291-302 0300-8177 399:1-2<291 2015 399 11010