caa a22 4500 606234101 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2247-4 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2247-4 Single-prolonged stress activates the transcription factor ATF6α branch of the unfolded protein response in rat neurons of dorsal raphe nucleus [Elektronische Daten] [Juhua Xie, Fang Han, Yuxiu Shi] In our previous studies, we have found that endoplasmic reticulum (ER) stress is associated with post-traumatic stress disorder (PTSD), however, the activation of ER stress sensors in PTSD remains unclear. ATF6 alpha (ATF6α) is an ER-membrane-bound transcription factor and functions as a critical sensor and regulator of ER stress in mammalian cells. The goal of this study is to detect whether there is activation of the transcription factor ATF6α branch of the unfolded protein response in the dorsal raphe nucleus neurons of the rats exposed to single-prolonged stress (SPS), which is a model employed extensively in PTSD study. Our results have demonstrated that SPS activated the ER transmembrane protein ATF6α via its cleavage; and induced the up-regulation of the downstream targets of ATF6α, the mRNA of XBP1 and GRP94. To the best of our knowledge, this is the first study to investigate the relationship between the ATF6α pathways and PTSD, and our results show that SPS activates the ATF6α branch of the ER stress response, which may be contributed to the pathogenesis of PTSD. Springer Science+Business Media New York, 2014 Single-prolonged stress nationallicence Post-traumatic stress disorder nationallicence Activating transcription factor 6α nationallicence X-box-binding protein 1 nationallicence Dorsal raphe nucleus nationallicence Xie Juhua Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut Han Fang Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut Shi Yuxiu Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 209-216 0300-8177 399:1-2<209 2015 399 11010 https://doi.org/10.1007/s11010-014-2247-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2247-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xie Juhua Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut NATIONALLICENCE 700 1- Han Fang Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut NATIONALLICENCE 700 1- Shi Yuxiu Laboratory of Post-Traumatic Stress Disorder, Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical Sciences College, China Medical University, 92 North 2nd Road, 110001, Shenyang, Liaoning Province, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 209-216 0300-8177 399:1-2<209 2015 399 11010