caa a22 4500 60623411X CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2239-4 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2239-4 MicroRNA-126 attenuates palmitate-induced apoptosis by targeting TRAF7 in HUVECs [Elektronische Daten] [Yi Wang, Feng Wang, Yan Wu, Li Zuo, Sumei Zhang, Qing Zhou, Wei Wei, Yuan Wang, Huaqing Zhu] The aim of the present study was to explore the role of miR-126 in palmitate-induced HUVECs apoptosis and the possible mechanisms. Palmitate inhibited miR-126 expression in HUVECs, increased reactive oxygen species (ROS) production, and induced apoptosis as determined by up-regulation of caspase-3 activity and DNA fragmentation. Overexpression of miR-126 decreased ROS production, TNF-α expression, and apoptosis in palmitate-stimulated HUVECs. In contrast, miR-126 antagomir enhanced palmitate-induced ROS production, TNF-α expression, and apoptosis. The induction of miR-126 correlated with a reduction in TRAF7. We further showed that miR-126 targeted and inhibited TRAF7 expression through target sites located in the 3′ untranslated region of TRAF7 mRNA. In concordance, miR-126 mimic reduced TRAF7 protein in HUVECs, whereas the inhibition of miR-126 increased it. This study demonstrates an anti-apoptotic role of miR-126 in HUVECs and identifies TRAF7 as a direct target of miR-126 in HUVECs. Springer Science+Business Media New York, 2014 miR-126 nationallicence Palmitate nationallicence ROS production nationallicence TRAF7 nationallicence Apoptosis nationallicence Wang Yi Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Wang Feng Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Wu Yan Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Zuo Li Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Zhang Sumei Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Zhou Qing Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Wei Wei Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Wang Yuan Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Zhu Huaqing Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 123-130 0300-8177 399:1-2<123 2015 399 11010 https://doi.org/10.1007/s11010-014-2239-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2239-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yi Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Feng Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wu Yan Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Zuo Li Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Sumei Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Qing Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wei Wei Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yuan Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Huaqing Institute of Clinical Pharmacology, Anhui Medical University, 230032, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 123-130 0300-8177 399:1-2<123 2015 399 11010