caa a22 4500 606234136 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2249-2 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2249-2 l -arginine stimulates CAT-1-mediated arginine uptake and regulation of inducible nitric oxide synthase for the growth of chick intestinal epithelial cells [Elektronische Daten] [Chao Yuan, Xiaoyun Zhang, Qiang He, Junming Li, Jianjun Lu, Xiaoting Zou] l-arginine (l-Arg) uptake is mediated by members of cationic amino acid transporter (CAT) family and may coincide with the induction of nitric oxide synthases (NOS). The present study was conducted to investigate the extracellular concentrations of l-Arg regulating the CAT-1, CAT-4 and inducible NOS (iNOS) in chick intestinal epithelial cells. The cells were cultured for 4days in Arg-free Dulbecco's modified Eagle's medium containing 10, 100, 200, 400, or 600μM l-Arg. Cell viability, nitric oxide (NO) concentrations, uptake and metabolism of l-[3H]-Arg as well as expression of CAT-1, CAT-4, and iNOS were determined. Our results showed that l-Arg enhances cell growth with a maximal response at 10-400μM. Addition of 100, 200, or 400μM l-Arg increased the l-[3H]-Arg uptake, which was associated with greater conversion of l-[3H]-citrulline and NO production in comparison with 10μM l-Arg group. Increasing extracellular concentrations of l-Arg from 10 to 400μM dose dependently increased the levels of CAT-1 mRNA and protein, while no effect on CAT-4 mRNA abundance was found. Furthermore, supplementation of 100, 200, or 400μM l-Arg upregulated the expression of iNOS mRNA, and the relative protein levels for iNOS in 200 and 400μM l-Arg groups were higher than those in 10 and 100μM l-Arg groups. Collectively, we conclude that the CAT-1 isoform plays a role in l-Arg uptake, and l-Arg-mediated elevation of NO via iNOS promotes the growth of chick intestinal epithelial cells. Springer Science+Business Media New York, 2014 Arginine nationallicence Arginine uptake nationallicence Cationic amino acid transporter nationallicence Chick intestinal epithelial cell nationallicence Inducible nitric oxide synthase nationallicence Yuan Chao Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut Zhang Xiaoyun Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut He Qiang Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut Li Junming Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut Lu Jianjun Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut Zou Xiaoting Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 229-236 0300-8177 399:1-2<229 2015 399 11010 https://doi.org/10.1007/s11010-014-2249-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2249-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yuan Chao Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Xiaoyun Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 700 1- He Qiang Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Li Junming Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Lu Jianjun Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zou Xiaoting Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 229-236 0300-8177 399:1-2<229 2015 399 11010