caa a22 4500 606234144 CHVBK 20210128101235.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2225-x doi (NATIONALLICENCE)springer-10.1007/s11010-014-2225-x Pioglitazone reduces lipid droplets in cholesterolosis of the gallbladder by increasing ABCA1 and NCEH1 expression [Elektronische Daten] [Jing-Min Wang, Dong Wang, Yu-Yan Tan, Gang Zhao, Zhen-Ling Ji] As a cholesterol-induced metabolic disease, cholesterolosis of the gallbladder is often resected clinically, which could lead to many complications. The histopathology of cholesterolosis is due to excessive lipid droplet accumulation in epithelial and subcutaneous tissues. The main components of lipid droplets are cholesterol esters (CEs). Removal of CEs from gallbladder epithelial cells (GBECs) is very important for maintaining intracellular cholesterol homeostasis and for treating cholesterol-related diseases. In this study, pioglitazone was used to reduce intracellular CEs. To further elucidate the mechanism, cholesterolosis GBECs were treated with pioglitazone, 22-(R)-hydroxycholesterol (a liver X receptor α (LXRα) agonist), or peroxisome proliferator-activated receptor gamma (PPARγ) siRNA. Western blotting for PPARγ, LXRα, ATP-binding cassette transporter A1 (ABCA1), and neutral cholesteryl ester hydrolase 1 (NCEH1) was performed. At length, cholesterol efflux to apoA-I was measured, and oil red O staining was used to visualize lipid droplet variations in cells. In conclusion, we observed that pioglitazone increased ABCA1 expression in an LXR-dependent manner and NCEH1 expression in an LXRα-independent manner, which mobilized CE hydrolysis and cholesterol efflux to reduce lipid droplet content in cholesterolosis GBECs. Our data provide a plausible alternative to human gallbladder cholesterolosis. Springer Science+Business Media New York, 2014 Cholesterolosis nationallicence Pioglitazone nationallicence PPARγ nationallicence LXRα nationallicence ABCA1 nationallicence NCEH1 nationallicence Wang Jing-Min Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut Wang Dong Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut Tan Yu-Yan Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut Zhao Gang Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut Ji Zhen-Ling Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 7-15 0300-8177 399:1-2<7 2015 399 11010 https://doi.org/10.1007/s11010-014-2225-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2225-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jing-Min Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Dong Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Tan Yu-Yan Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Zhao Gang Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Ji Zhen-Ling Department of General Surgery, Institute for Minimally Invasive Surgery, Zhongda Hospital Southeast University, Medical School of Southeast University, 87 Ding Jia Qiao, 210009, Nanjing, Jiangsu, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 7-15 0300-8177 399:1-2<7 2015 399 11010