caa a22 4500 606234187 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2231-z doi (NATIONALLICENCE)springer-10.1007/s11010-014-2231-z Nicotine-induced upregulation of VCAM-1, MMP-2, and MMP-9 through the α7-nAChR-JNK pathway in RAW264.7 and MOVAS cells [Elektronische Daten] [Zong-Zhuang Li, Zhen-Zhen Guo, Zhi Zhang, Qun-An Cao, Ya-Juan Zhu, Hua-Li Yao, Li-Li Wu, Qiu-Yan Dai] The ability of nicotine to induce aortic aneurysms has been shown in animal models; however, its underlying mechanisms remain elusive. In the present experiment, both the RAW264.7 and MOVAS cell lines were employed to examine the nicotine-induced modulation of VCAM-1, MMP-2, and MMP-9 expressions in macrophages and vascular smooth muscle cells. Our results showed that nicotine concentrations of both 0.5 and 5ng/ml induced VCAM-1, MMP-2, and MMP-9 upregulation, while a concentration of 50ng/ml had a slight inhibitory effect and a concentration of 500ng/ml showed a significant inhibitory effect. When cells were pretreated with either SP600125 (JNK inhibitor) or PNU-282987 (α7-nAChR agonist) prior to nicotine exposure, the nicotine-induced upregulation of VCAM-1, MMP-2, MMP-9, and p-JNK was suppressed, with a joint treatment producing a more significant inhibitory effect. Moreover, PNU-282987 had a comparable inhibitory effect on VCAM-1, MMP-2, and MMP-9 expressions and JNK activation via phosphorylation as did SP600125. In conclusion, nicotine-induced VCAM-1, MMP-2, and MMP-9 expressions occur in a dose-dependent fashion in both of the cell lines tested. Furthermore, the nicotine exposure equivalent to plasma levels found in regular smokers can augment VCAM-1, MMP-2, and MMP-9 expressions through the α7-nAChR-JNK pathway. Springer Science+Business Media New York, 2014 Nicotine nationallicence JNK nationallicence Nicotinic acetylcholine receptors nationallicence Vascular cell adhesion molecular nationallicence Matrix metalloproteinase nationallicence Li Zong-Zhuang Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Guo Zhen-Zhen Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Zhang Zhi Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Cao Qun-An Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Zhu Ya-Juan Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Yao Hua-Li Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Wu Li-Li Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Dai Qiu-Yan Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 49-58 0300-8177 399:1-2<49 2015 399 11010 https://doi.org/10.1007/s11010-014-2231-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2231-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zong-Zhuang Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Guo Zhen-Zhen Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Zhi Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Cao Qun-An Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Zhu Ya-Juan Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Yao Hua-Li Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Wu Li-Li Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 700 1- Dai Qiu-Yan Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, 200080, Shanghai, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 49-58 0300-8177 399:1-2<49 2015 399 11010