caa a22 4500 606234217 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2241-x doi (NATIONALLICENCE)springer-10.1007/s11010-014-2241-x LAP1 is a crucial protein for the maintenance of the nuclear envelope structure and cell cycle progression [Elektronische Daten] [Mariana Santos, Patrícia Costa, Filipa Martins, Edgar da Cruz e Silva, Odete da Cruz e Silva, Sandra Rebelo] Cell division in eukaryotes requires the disassembly of the nuclear envelope (NE) at the beginning of mitosis and its reassembly at the end of mitosis. These processes are complex and involve coordinated steps where NE proteins have a crucial role. Lamina-associated polypeptide 1 (LAP1) is an inner nuclear membrane protein that has been associated with cell cycle events. In support of this role, LAP1 has been implicated in the regulation of the NE reassembly and assembly of the mitotic spindle during mitosis. In this study, we demonstrated that LAP1 intracellular levels vary during the cell cycle in SH-SY5Y cells, and that LAP1 is highly phosphorylated during mitosis. It is also clear that LAP1 co-localized with acetylated α-tubulin in the mitotic spindle and with γ-tubulin in centrosomes (main microtubule organizing center) in mitotic cells. Moreover, LAP1 knockdown resulted in decreased number of mitotic cells and decreased levels of acetylated α-tubulin (marker of microtubules stability) and lamin B1. Additionally, it was possible to determine that LAP1 is important for centrosome positioning near the NE. These findings place LAP1 at a key position to participate in the maintenance of the NE structure and progression of the cell cycle. Springer Science+Business Media New York, 2014 Lamina-associated polypeptide 1 nationallicence Nuclear envelope nationallicence Cell cycle nationallicence Protein phosphorylation nationallicence Centrosome nationallicence Santos Mariana Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut Costa Patrícia Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut Martins Filipa Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut da Cruz e Silva Edgar Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut da Cruz e Silva Odete Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut Rebelo Sandra Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 143-153 0300-8177 399:1-2<143 2015 399 11010 https://doi.org/10.1007/s11010-014-2241-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2241-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santos Mariana Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Costa Patrícia Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Martins Filipa Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- da Cruz e Silva Edgar Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- da Cruz e Silva Odete Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Rebelo Sandra Neuroscience Laboratory, Health Sciences Department, Centre for Cell Biology, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 143-153 0300-8177 399:1-2<143 2015 399 11010