caa a22 4500 606234225 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2229-6 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2229-6 Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast [Elektronische Daten] [Luka Andrisic, Emma Collinson, Oksana Tehlivets, Eleonora Perak, Tomislav Zarkovic, Ian Dawes, Neven Zarkovic, Ana Cipak Gasparovic] Pathophysiology of polyunsaturated fatty acids (PUFAs) is associated with aberrant lipid and oxygen metabolism. In particular, under oxidative stress, PUFAs are prone to autocatalytic degradation via peroxidation, leading to formation of reactive aldehydes with numerous potentially harmful effects. However, the pathological and compensatory mechanisms induced by lipid peroxidation are very complex and not sufficiently understood. In our study, we have used yeast capable of endogenous PUFA synthesis in order to understand the effects triggered by PUFA accumulation on cellular physiology of a eukaryotic organism. The mechanisms induced by PUFA accumulation in S. cerevisiae expressing Hevea brasiliensis Δ12-fatty acid desaturase include down-regulation of components of electron transport chain in mitochondria as well as up-regulation of pentose-phosphate pathway and fatty acid β-oxidation at the transcriptional level. Interestingly, while no changes were observed at the transcriptional level, activities of two important enzymatic antioxidants, catalase and glutathione-S-transferase, were altered in response to PUFA accumulation. Increased intracellular glutathione levels further suggest an endogenous oxidative stress and activation of antioxidative defense mechanisms under conditions of PUFA accumulation. Finally, our data suggest that PUFA in cell membrane causes metabolic changes which in turn lead to adaptation to endogenous oxidative stress. Springer Science+Business Media New York, 2014 PUFA nationallicence Desaturase nationallicence ROS nationallicence Fatty acid β-oxidation nationallicence Catalase nationallicence Andrisic Luka Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut Collinson Emma Discipline of Anatomy and Histology, School of Medical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Tehlivets Oksana Institute of Molecular Biosciences, Lipidomics Research Center Graz, University of Graz, Graz, Austria aut Perak Eleonora Veterinary Institute, Zagreb, Croatia aut Zarkovic Tomislav Molecular Biosciences Study Program on Bioinformatics/Zagrebacka banka, Zagreb, Croatia aut Dawes Ian School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia aut Zarkovic Neven Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut Cipak Gasparovic Ana Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 27-37 0300-8177 399:1-2<27 2015 399 11010 https://doi.org/10.1007/s11010-014-2229-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2229-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Andrisic Luka Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Collinson Emma Discipline of Anatomy and Histology, School of Medical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Tehlivets Oksana Institute of Molecular Biosciences, Lipidomics Research Center Graz, University of Graz, Graz, Austria aut NATIONALLICENCE 700 1- Perak Eleonora Veterinary Institute, Zagreb, Croatia aut NATIONALLICENCE 700 1- Zarkovic Tomislav Molecular Biosciences Study Program on Bioinformatics/Zagrebacka banka, Zagreb, Croatia aut NATIONALLICENCE 700 1- Dawes Ian School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 700 1- Zarkovic Neven Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut NATIONALLICENCE 700 1- Cipak Gasparovic Ana Rudjer Boskovic Institute, Bijenicka 54, 10000, Zagreb, Croatia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 27-37 0300-8177 399:1-2<27 2015 399 11010