caa a22 4500 606234233 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2236-7 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2236-7 Enhanced ROS production and oxidative damage in subcutaneous white adipose tissue mitochondria in obese and type 2 diabetes subjects [Elektronische Daten] [Mrittika Chattopadhyay, Vineet Khemka, Gargi Chatterjee, Anirban Ganguly, Satinath Mukhopadhyay, Sasanka Chakrabarti] Oxidative stress in the insulin target tissues has been implicated in the pathophysiology of type 2 diabetes. The study has examined the oxidative stress parameters in the mitochondria of subcutaneous white adipose tissue from obese and non-obese subjects with or without type 2 diabetes. An accumulation of protein carbonyls, fluorescent lipid peroxidation products, and malondialdehyde occurs in the adipose tissue mitochondria of obese type 2 diabetic, non-diabetic obese, and non-obese diabetic subjects with the maximum increase noticed in the obese type 2 diabetes patients and the minimum in non-obese type 2 diabetics. The mitochondria from obese type 2 diabetics, non-diabetic obese, and non-obese type 2 diabetics also produce significantly more reactive oxygen species (ROS) in vitro compared to those of controls, and apparently the mitochondrial ROS production rate in each group is proportional to the respective load of oxidative damage markers. Likewise, the mitochondrial antioxidant enzymes like superoxide dismutase and glutathione peroxidase show decreased activities most markedly in obese type 2 diabetes subjects and to a lesser degree in non-obese type 2 diabetes or non-diabetic obese subjects in comparison to control. The results imply that mitochondrial dysfunction with enhanced ROS production may contribute to the metabolic abnormality of adipose tissue in obesity and diabetes. Springer Science+Business Media New York, 2014 Diabetes nationallicence Obesity nationallicence Oxidative stress nationallicence Adipose tissue nationallicence Mitochondrial dysfunction nationallicence Chattopadhyay Mrittika Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Khemka Vineet Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Chatterjee Gargi Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Ganguly Anirban Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Mukhopadhyay Satinath Department of Endocrinology and Metabolism, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Chakrabarti Sasanka Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 95-103 0300-8177 399:1-2<95 2015 399 11010 https://doi.org/10.1007/s11010-014-2236-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2236-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chattopadhyay Mrittika Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Khemka Vineet Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Chatterjee Gargi Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Ganguly Anirban Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Mukhopadhyay Satinath Department of Endocrinology and Metabolism, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Chakrabarti Sasanka Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244, Acharya J. C. Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 95-103 0300-8177 399:1-2<95 2015 399 11010