caa a22 4500 606234241 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2251-8 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2251-8 LSM14A inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication by activating IFN-β signaling pathway in Marc-145 [Elektronische Daten] [Zhenhong Li, Rui Chen, Jinhua Zhao, Ziyu Qi, Likai Ji, Yueran Zhen, Bang Liu] Porcine reproductive and respiratory syndrome virus (PRRSV) is considered as a significant contributor to porcine reproductive and respiratory syndrome, one of the most economically important diseases for the pig industry worldwide. Emerging evidence indicates that pattern recognition receptors play key roles in recognizing pathogen-associated molecular patterns. In the present study, we investigated the effects of a novel pattern recognition receptor LSM14A in regulating PRRSV replication. Results in Marc-145 cells and porcine alveolar macrophages (PAMs) indicated that overexpression of porcine LSM14A effectively inhibited the replication of PRRSV, and knockdown of LSM14A by siRNA enhanced the replication of PRRSV. Mechanistically, LSM14A up-regulated the activities of IFN-β and ISRE promoters, enhanced the production of IFN-β, RIG-I, and ISGs, and inhibited the production of the inflammatory cytokines of TNF-α and IL-6 mRNA. Additionally, the expression pattern of LSM14A during the infection of PRRSV in Tongcheng and Large White pigs was suppressed by the PRRSV challenge. Taken together, our results suggest that LSM14A is an important PRR that inhibits PPRSV replication by inducing IFN-β signaling and restraining inflammatory responses. Furthermore, the down-regulation of LSM14A by PRRSV might represent an important mechanism by which PRRSV invades the host. Our study sheds light on the possibility of developing a new strategy to control this disease. Springer Science+Business Media New York, 2014 LSM14A nationallicence PRRSV nationallicence PRRs nationallicence IFN-β nationallicence Antiviral nationallicence Marc-145 cells nationallicence PAMs nationallicence Li Zhenhong Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Chen Rui Department of Pharmacology and Cancer Biology, Duke University Medical Center, 27710, Durham, NC, USA aut Zhao Jinhua Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Qi Ziyu Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Ji Likai Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Zhen Yueran Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Liu Bang Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 247-256 0300-8177 399:1-2<247 2015 399 11010 https://doi.org/10.1007/s11010-014-2251-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2251-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zhenhong Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Chen Rui Department of Pharmacology and Cancer Biology, Duke University Medical Center, 27710, Durham, NC, USA aut NATIONALLICENCE 700 1- Zhao Jinhua Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Qi Ziyu Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Ji Likai Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhen Yueran Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Liu Bang Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 247-256 0300-8177 399:1-2<247 2015 399 11010