caa a22 4500 606234292 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2226-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2226-9 Transcript expression of the freeze responsive gene fr10 in Rana sylvatica during freezing, anoxia, dehydration, and development [Elektronische Daten] [K. Sullivan, K. Biggar, K. Storey] Freeze tolerance is a critical winter survival strategy for the wood frog, Rana sylvatica. In response to freezing, a number of genes are upregulated to facilitate the survival response. This includes fr10, a novel freeze-responsive gene first identified in R. sylvatica. This study analyzes the transcriptional expression of fr10 in seven tissues in response to freezing, anoxia, and dehydration stress, and throughout the Gosner stages of tadpole development. Transcription of fr10 increased overall in response to 24h of freezing, with significant increases in expression detected in testes, heart, brain, and lung when compared to control tissues. When exposed to anoxia; heart, lung, and kidney tissues experienced a significant increase, while the transcription of fr10 in response to 40% dehydration was found to significantly increase in both heart and brain tissues. An analysis of the transcription of fr10 throughout the development of the wood frog showed a relatively constant expression; with slightly lower transcription levels observed in two of the seven Gosner stages. Based on these results, it is predicted that fr10 has multiple roles depending on the needs and stresses experienced by the wood frog. It has conclusively been shown to act as a cryoprotectant, with possible additional roles in anoxia, dehydration, and development. In the future, it is hoped that further knowledge of the mechanism of action of FR10 will allow for increased stress tolerance in human cells and tissues. Springer Science+Business Media New York, 2014 Cryobiology nationallicence Frog nationallicence Anti-freeze protein nationallicence Protein structure nationallicence Sullivan K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut Biggar K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut Storey K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 17-25 0300-8177 399:1-2<17 2015 399 11010 https://doi.org/10.1007/s11010-014-2226-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2226-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sullivan K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Biggar K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Storey K. Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 17-25 0300-8177 399:1-2<17 2015 399 11010