caa a22 4500 606234314 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2240-y doi (NATIONALLICENCE)springer-10.1007/s11010-014-2240-y Type-2 cannabinoid receptor regulates proliferation, apoptosis, differentiation, and OPG/RANKL ratio of MC3T3-E1 cells exposed to Titanium particles [Elektronische Daten] [Shang Qiu, Fengchao Zhao, Xianye Tang, Fang Pei, Hongyan Dong, Liang Zhu, Kaijin Guo] The type-2 cannabinoid receptor (CB2) is expressed in osteoblasts and plays a role in bone metabolism through regulation on bone mass and bone turnover, but the functional importance of CB2 in osteoblasts under Titanium (Ti) stimulation is incompletely understood. This study aimed to investigate the CB2 expression in osteoblasts under Ti stimulation and the effects of CB2 activation on proliferation, apoptosis, differentiation, mineralization, OPG, and RANKL expression of MC3T3-E1 cells exposed to Ti particles. MC3T3-E1 cells were incubated in the presence of Ti particles with or without CB2-specific agonist HU-308 and antagonist SR144528. Ti particles treatment obviously induced the CB2 expression in MC3T3-E1 cells, and reduced the cell survival in a dose- and time-dependent manner (p<0.05). Addition of HU-308 could dose-dependently alleviate the Ti-induced decrease of cell survival (p<0.05). The flow cytometry assay showed that comparing with the control group, the apoptosis rate and caspase-3 activity in the Ti group were significantly elevated (p<0.05), which could be alleviated by HU-308. Moreover, HU-308 effectively attenuated the decrease of cell mineralization capability, alkaline phosphates (ALP) and osteocalcin activity, and increase of OPG/RANKL ratio induced by Ti particles treatment (p<0.05). These effects were partially counteracted by combined treatment of CB2 antagonist SR144528 (p<0.05). In conclusion, CB2 activation has a favorable inhibitory effect on Ti-induced reactions in MC3T3-E1 cell through modulating proliferation, apoptosis, differentiation, and RANKL expression. These findings suggest that activation of CB2 might be an effective therapeutic strategy to promote bone formation and reduce bone dissolution. Springer Science+Business Media New York, 2014 CB2 nationallicence HU-308 nationallicence Titanium nationallicence MC3T3-E1 nationallicence Proliferation nationallicence Apoptosis nationallicence OPG nationallicence RANKL nationallicence Qiu Shang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Zhao Fengchao Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Tang Xianye Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Pei Fang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Dong Hongyan Institute of Neurobiology, Xuzhou Medical College, 223000, Jiangsu, China aut Zhu Liang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Guo Kaijin Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 131-141 0300-8177 399:1-2<131 2015 399 11010 https://doi.org/10.1007/s11010-014-2240-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2240-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiu Shang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Zhao Fengchao Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Tang Xianye Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Pei Fang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Dong Hongyan Institute of Neurobiology, Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Zhu Liang Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 700 1- Guo Kaijin Orthopaedic Department of Affiliated Hospital of Xuzhou Medical College, 223000, Jiangsu, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 131-141 0300-8177 399:1-2<131 2015 399 11010