caa a22 4500 606234330 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2252-7 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2252-7 Silver nanoparticle-induced oxidative stress-dependent toxicity in Sprague-Dawley rats [Elektronische Daten] [Anita Patlolla, Diahanna Hackett, Paul Tchounwou] Due to the intensive commercial application of silver nanoparticles (Ag-NPs), their health risk assessment is of great importance. For acute toxicity evaluation of orally administered Ag-NPs, induction of reactive oxygen species (ROS), activity of liver function enzymes [(alanine (ALT/GPT), aspartate (AST/GOT), alkaline phosphatase (ALP)], concentration of lipid hydroperoxide (LHP), comet assay, and histopathology of liver in the rat model were performed. Four groups of five male rats were orally administered Ag-NPs, once a day for five days with doses of 5, 25, 50, 100mg/kg, body weight. A control group was also made of five rats. Blood and liver were collected 24h after the last treatment following standard protocols. Ag-NPs exposure increased the induction of ROS, activities of the liver enzymes (ALT, AST, ALP), concentration of lipid hydroperoxide (LHP), tail migration, and morphological alterations of the liver tissue in exposed groups compared to control. The highest two doses, 50 and 100mg/kg showed statistically significant (p<0.05) increases in ROS induction, ALT, AST, ALP activity, LHP concentration, DNA damage, and morphological alterations of liver compared to control. Based on these results, it is suggested that short-term administration of high doses of Ag-NP may cause organ toxicity and oxidative stress. Springer Science+Business Media New York, 2014 Silver nanoparticles nationallicence Hepatotoxicity nationallicence Reactive oxygen species nationallicence Serum aminotransferases (alanine aspartate) nationallicence Alkaline phosphatase nationallicence Lipid hydroperoxide nationallicence Histopathology nationallicence Comet assay and Sprague-Dawley rats nationallicence Patlolla Anita NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut Hackett Diahanna NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut Tchounwou Paul NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 257-268 0300-8177 399:1-2<257 2015 399 11010 https://doi.org/10.1007/s11010-014-2252-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2252-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patlolla Anita NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut NATIONALLICENCE 700 1- Hackett Diahanna NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut NATIONALLICENCE 700 1- Tchounwou Paul NIH-Center for Environmental Health, College of Science Engineering and Technology, Jackson State University, Jackson, MS, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 257-268 0300-8177 399:1-2<257 2015 399 11010