caa a22 4500 606234357 CHVBK 20210128101236.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2235-8 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2235-8 Thyroid hormone deiodinases D1, D2, and D3 are expressed in human endothelial dermal microvascular line: effects of thyroid hormones [Elektronische Daten] [Laura Sabatino, Valter Lubrano, Silvana Balzan, Claudia Kusmic, Serena Del Turco, Giorgio Iervasi] Endothelial system acts as a large endocrine organ in the human body; however, little is still known about the regulative role of THs on endothelial cells. Aim of the present study was to investigate the expression of the TH deiodinases (D1, D2, and D3) and TH receptors (TRα1, TRα2, and TRβ1) in an endothelial microvascular cultured cell model (HMEC-1), after stimulation with triiodothyronine (T3, 10-100nM), thyroxine (T4, 10-100nM), and reverse T3 (rT3, 1-10nM). DIO1 was significantly inhibited by T4 at 10 and 100nM (p<0.001). rT3 significantly inhibited DIO1 at 1nM concentration (p<0.01) and stimulated DIO1 at 10nM dosage (p<0.001). T4 and rT3 significantly inhibited DIO2 at all concentrations. DIO3 was induced at 100nM T3 (p<0.05) and 100nM rT3 (p<0.01), and TRα1 and TRα2 mRNAs were significantly increased after 100nM T3 treatment (p<0.05) and decreased after 1 and 10nM rT3 (p<0.05). TRβ1 was significantly increased by all THs at different concentrations: 10nM T3 and 100nM T3 (p<0.05), 1nM rT3 (p<0.001), and 100nM T4 (p<0.01). D1 and D2 protein levels were evaluated, but no significant difference was observed for any hormonal treatment. For the first time, we found that the TH deiodinases and receptors are expressed in endothelial HMEC-1 cells. These findings might be of significant clinical relevance, given the important regulatory role of the endothelium as first barrier to the bloodstream. Springer Science+Business Media New York, 2014 Thyroid hormones nationallicence Deiodinases nationallicence Endothelial cells nationallicence Sabatino Laura Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut Lubrano Valter Fondazione G. Monasterio CNR-Regione Toscana, Via Moruzzi 1, 56124 Pisa, Italy aut Balzan Silvana Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut Kusmic Claudia Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut Del Turco Serena Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut Iervasi Giorgio Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 87-94 0300-8177 399:1-2<87 2015 399 11010 https://doi.org/10.1007/s11010-014-2235-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2235-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sabatino Laura Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut NATIONALLICENCE 700 1- Lubrano Valter Fondazione G. Monasterio CNR-Regione Toscana, Via Moruzzi 1, 56124 Pisa, Italy aut NATIONALLICENCE 700 1- Balzan Silvana Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut NATIONALLICENCE 700 1- Kusmic Claudia Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut NATIONALLICENCE 700 1- Del Turco Serena Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut NATIONALLICENCE 700 1- Iervasi Giorgio Institute of Clinical Physiology, National Research Council (CNR), Via Moruzzi 1, 56124, Pisa, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 399/1-2(2015-01-01), 87-94 0300-8177 399:1-2<87 2015 399 11010