caa a22 4500 606234365 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2395-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2395-1 Effects of 5′-azacytidine and alendronate on a hepatocellular carcinoma cell line: a proteomics perspective [Elektronische Daten] [Amber Ilyas, Zehra Hashim, Shamshad Zarina] Hepatocellular carcinoma (HCC) is the third leading cause of cancer related deaths around the world. Due to late diagnosis and development of drug resistance in patients suffering from HCC, development of more effective therapeutic strategies is inevitable. The aim of this study was to evaluate the combined apoptotic effect of 5′-Azacytidine (5′-AzaC) and alendronate (ALN) on Huh-7 HCC cell line and to explore differential expression at genomics and proteomics level. Incubation of HCC cell line with 5′-AzaC alone showed cell death in a time and dose dependent manner while in combination with ALN, increased cytotoxicity was observed. Up-regulation of CASP7(Caspase7) and LZTS1 (leucine zipper, putative tumor suppressor 1) and down-regulation of DNMT1(DNA (cytosine-5-)-methyltransferase 1) was noted in treated cells. Proteomic studies on the treated cells revealed altered expression of different proteins including peroxiredoxin 2 (Prx2), Annexin 5 (Anx5), Rho GTPase activating protein (RhoGAP), Nuclear factor-kappa B (NF-kB), tumor necrosis factor alpha-induced protein (TNF), triosephosphate isomerase (TPI), Glutathione S transferase (GSTP1) and Heat shock protein60 (HSP60). Our study demonstrated the cytotoxic effect of 5′-AzaC and ALN drug combination on Huh-7 HCC cells suggesting such combinations may be explored as a possible therapeutic approach. Current study revealed that Huh-7 HCC cells are sensitive to 5′-AzaC and ALN drug combination and such combination approaches could lead to the development of new therapeutic strategies. Furthermore, we also report the expression of Anx5 exclusively in untreated cancerous cell line indicating the possibility of being used as a potential therapeutic target and biomarker. Springer Science+Business Media New York, 2015 5′-Azacytidine nationallicence Alendronate nationallicence Hepatocellular carcinoma nationallicence Huh 7 cell line nationallicence Ilyas Amber National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut Hashim Zehra National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut Zarina Shamshad National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 53-61 0300-8177 405:1-2<53 2015 405 11010 https://doi.org/10.1007/s11010-015-2395-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2395-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ilyas Amber National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Hashim Zehra National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Zarina Shamshad National Center for Proteomics, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 53-61 0300-8177 405:1-2<53 2015 405 11010