caa a22 4500 606234373 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2407-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2407-1 Effect of amino acid substitution in the staphylococcal peptides warnericin RK and PSMα on their anti- Legionella and hemolytic activities [Elektronische Daten] [Adrienne Marchand, Jacques Augenstreich, Clémence Loiseau, Julien Verdon, Sophie Lecomte, Jean-Marc Berjeaud] Warnericin RK from Staphylococcus warneri and PSMα from Staphylococcus epidermidis are anti-Legionella peptides which were differently classified in a previous study according to their mode of action. Indeed, warnericin RK is highly hemolytic with a bactericidal mode of action, whereas PSMα is poorly hemolytic with a bacteriostatic mode of action toward L. pneumophila. In order to find anti-Legionella peptides which are not hemolytic, a collection of peptides varying in sequence from warnericin RK to PSMα were designed and synthesized, and their anti-Legionella activities, in terms of growth inhibition, permeabilization, and bactericidal effect, as well as their hemolytic activities, were measured and compared. The results showed that some residues, at position 14 for both peptides for instance, were of major importance for bactericidal and hemolytic activities. Springer Science+Business Media New York, 2015 Legionella pneumophila nationallicence Anti- Legionella peptides nationallicence Hemolysis nationallicence Structure-function nationallicence Marchand Adrienne Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Augenstreich Jacques Chimie Biologie des Membranes et Nanoobjets, CBMN, CNRS UMR5248-Université Bordeaux1, 14 allée Geoffroy de Saint Hilaire, 33600, Pessac, France aut Loiseau Clémence Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Verdon Julien Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Lecomte Sophie Chimie Biologie des Membranes et Nanoobjets, CBMN, CNRS UMR5248-Université Bordeaux1, 14 allée Geoffroy de Saint Hilaire, 33600, Pessac, France aut Berjeaud Jean-Marc Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 159-167 0300-8177 405:1-2<159 2015 405 11010 https://doi.org/10.1007/s11010-015-2407-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2407-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marchand Adrienne Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Augenstreich Jacques Chimie Biologie des Membranes et Nanoobjets, CBMN, CNRS UMR5248-Université Bordeaux1, 14 allée Geoffroy de Saint Hilaire, 33600, Pessac, France aut NATIONALLICENCE 700 1- Loiseau Clémence Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Verdon Julien Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Lecomte Sophie Chimie Biologie des Membranes et Nanoobjets, CBMN, CNRS UMR5248-Université Bordeaux1, 14 allée Geoffroy de Saint Hilaire, 33600, Pessac, France aut NATIONALLICENCE 700 1- Berjeaud Jean-Marc Université de Poitiers, Ecologie & Biologie des Interactions, CNRS UMR7267 Equipe Microbiologie de l'Eau, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 159-167 0300-8177 405:1-2<159 2015 405 11010