caa a22 4500 606234381 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2403-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2403-5 MicroRNA-221/222 regulate ox-LDL-induced endothelial apoptosis via Ets-1/p21 inhibition [Elektronische Daten] [Bing Qin, Yuze Cao, Huan Yang, Bo Xiao, Zhengqi Lu] Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play an essential role in atherosclerosis. MicroRNAs (miRNAs) are a class of short non-coding RNAs, acting as posttranscriptional regulators of protein-coding genes involved in vascular cell biology. MiRNA-221 and miRNA-222 (miR-221/222) are known to be involved in the regulation of endothelial inflammation and angiogenesis. However, the function of miR-221/222 in ox-LDL-induced ECs apoptosis and atherosclerosis is still unknown. Here, we showed that miR-221/222 expression was markedly down-regulated in ox-LDL-induced apoptotic human umbilical cord vein endothelial cells. MiR-221/222 inhibition enhanced apoptosis in ECs, whereas over-expression of miR-221/222 could partly alleviate apoptotic cell death mediated by ox-LDL through suppression of Ets-1 and its downstream target p21. These findings suggest that manipulation of the miR-221/222-Ets-1-p21 pathway may offer a novel strategy for treatment of endothelial apoptosis and atherosclerosis. Springer Science+Business Media New York, 2015 microRNA-221/222 nationallicence HUVECs nationallicence Apoptosis nationallicence Ets-1 nationallicence Atherosclerosis nationallicence Qin Bing Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, 510630, Guangzhou, Guangdong, People's Republic of China aut Cao Yuze Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut Yang Huan Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut Xiao Bo Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut Lu Zhengqi Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, 510630, Guangzhou, Guangdong, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 115-124 0300-8177 405:1-2<115 2015 405 11010 https://doi.org/10.1007/s11010-015-2403-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2403-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Bing Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, 510630, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Cao Yuze Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Yang Huan Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Bo Department of Neurology, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Lu Zhengqi Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, 510630, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 115-124 0300-8177 405:1-2<115 2015 405 11010