caa a22 4500 606234411 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2411-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2411-5 Hyperhomocysteinemia regulated SCF expression in cultured cardiomyocytes via modulation of NF-κB activities [Elektronische Daten] [Xia Zhao, Dong Kuang, Yuping Duan, Guixiang Xiao, Juan Ni, Yaqi Duan, Guoping Wang] Hyperhomocysteinemia (HHcy) is an important, independent risk factor for coronary artery disease, especially for the myocardial infarction. Our previous study has shown that myocardial stem cell factor (SCF) mediated cardiac stem cells migration, which was involved in cardiac repair. However, it is not clear regarding the action of HHcy on the expression of SCF in cardiomyocytes. In the present study, cultured neonatal rat cardiomyocytes were treated with 20, 50, or 100μM homocysteine (Hcy) for 5h. Results showed an significantly increase of SCF expression with 20-50μM Hcy incubation, which matched with elevated nuclear factor-kappaB (NF-κB) activities. Treatment with NF-κB inhibitor N-acetylcysteine significantly inhibited the increase of SCF. Nevertheless, 100μM Hcy markedly decreased the expression of SCF, which was in accordance with the suppression of NF-κB activities. The present study indicated that HHcy regulated the expression of SCF in a concentration-dependent manner via modulation of NF-κB activities. Thus, HHcy may increase the risk for cardiovascular diseases not only by causing endothelial dysfunction but also by directly exerting detrimental effects on cardiomyocytes. Springer Science+Business Media New York, 2015 Hyperhomocysteinemia nationallicence Stem cell factor nationallicence NF-κB nationallicence Myocardial infarction nationallicence Zhao Xia Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Kuang Dong Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Duan Yuping Department of Vitreous and Retinal Disease, Wuhan Eyegood Ophthalmic Hospital, 430019, Wuhan, China aut Xiao Guixiang Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Ni Juan Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Duan Yaqi Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Wang Guoping Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 197-203 0300-8177 405:1-2<197 2015 405 11010 https://doi.org/10.1007/s11010-015-2411-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2411-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Xia Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Kuang Dong Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Duan Yuping Department of Vitreous and Retinal Disease, Wuhan Eyegood Ophthalmic Hospital, 430019, Wuhan, China aut NATIONALLICENCE 700 1- Xiao Guixiang Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Ni Juan Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Duan Yaqi Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Wang Guoping Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Da Dao, 430030, Wuhan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 197-203 0300-8177 405:1-2<197 2015 405 11010