caa a22 4500 60623442X CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2391-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2391-5 Association of estrogen receptor β and estrogen-related receptor α gene polymorphisms with bone mineral density in postmenopausal women [Elektronische Daten] [Amira Shoukry, Sally Shalaby, Rasha Etewa, Hanan Ahmed, Hossam Abdelrahman] The aim of the study was to investigate the possible association of AluI and RsaI polymorphisms of estrogen receptor β (ER-β) gene and 23-bp nucleotide repeat polymorphism of estrogen-related receptor α (ERRα) gene with bone mineral density (BMD) in postmenopausal Egyptian women. Two-hundred postmenopausal osteoporotic women as cases and 180 healthy age-matched postmenopausal women as controls were genotyped by PCR fragment length polymorphism for AluI, allele-specific PCR for RsaI, and by sizing of PCR products on agarose gels for ERRα repeats. sRANKL levels were estimated by ELISA. BMD measurements for spine and femoral neck were performed by dual energy X-ray absorptiometry. A significant difference between women with osteoporosis and controls regarding allele and genotype distributions of AluI G/A (OR 2.37, 95% CI 1.77-3.18 and p<0.001 for A allele) and ERRα polymorphisms (for the two repeats allele OR 2.08, 95% CI 1.09-4.00, and p=0.02). Osteoporotic women with the AluI AA + GA genotype or with the EERα 2,2 genotype had significantly lower BMD than did women with the other genotypes. Moreover, there was a significant increase of the mean values of sRANKL in carriers of AluI A, RsaI A alleles and in patients having 2,2 genotypes of ERRα (p<0.001, p<0.001, p=0.02, respectively). We demonstrated an association of ER-β AluI G/A and ERRα 23-repeats polymorphisms with BMD in postmenopausal Egyptian women. A possible effect of ER-β and ERRα polymorphisms on the levels of sRANKL was estimated. Springer Science+Business Media New York, 2015 Osteoporosis nationallicence Estrogen receptor β nationallicence Estrogen-related receptor α polymorphisms nationallicence RANKL nationallicence Shoukry Amira Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Shalaby Sally Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Etewa Rasha Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Ahmed Hanan Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Abdelrahman Hossam Radiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 23-31 0300-8177 405:1-2<23 2015 405 11010 https://doi.org/10.1007/s11010-015-2391-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2391-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shoukry Amira Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- Shalaby Sally Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- Etewa Rasha Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- Ahmed Hanan Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- Abdelrahman Hossam Radiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 23-31 0300-8177 405:1-2<23 2015 405 11010