caa a22 4500 606234454 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2417-z doi (NATIONALLICENCE)springer-10.1007/s11010-015-2417-z Role of NF-κB activation and VEGF gene polymorphisms in VEGF up regulation in non-proliferative and proliferative diabetic retinopathy [Elektronische Daten] [Subhadip Choudhuri, Imran Chowdhury, Shibali Das, Deep Dutta, Avijit Saha, Rajarshi Sarkar, Lakshmi Mandal, Satinath Mukherjee, Basudev Bhattacharya] The present study was aimed to investigate the relation between nuclear factor kappa beta (NFκB) activation and downstream up-regulation of vascular endothelial growth factor (VEGF) in diabetic retinopathy (DR). Moreover the study was intended to evaluate the role of VEGF gene single nucleotide polymorphisms (SNPs) in DR occurrence and to investigate the functional relevance of VEGF gene SNPs in terms of VEGF expression in DR. Serum level of VEGF, VEGF R1 (receptor 1), VEGF R 2 (receptor 2) and NFκB (p50/65) activity was measured by enzyme linked immune sorbent assay. Genotyping and allelic composition of different SNPs i.e., rs2010963, rs3025039, rs1570360 and rs 2071559 were investigated by Taqman SNP genotyping assay. VEGF, NFκB p50/p65, and VEGF R1 & R2 gene expressions were quantified by real time quantitative polymerase chain reaction. Increased NFκB p50/p65 activity and expressions were observed in non proliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR) subjects compared to type 2 diabetes mellitus without retinopathy (DNR) group. Significantly elevated levels of serum VEGF and highest VEGF expression were found among PDR subjects compared to DNR or NPDR subjects. CC genotype and C allele of rs2010963 and TT genotype and T allele of rs3025039 were significantly over represented among PDR subjects compared to DNR group. Increased activation of NFκβ in NPDR and PDR subjects might involve increased up regulation of VEGF. VEGF SNPs i.e., rs2010963 C allele and rs3025039 T allele might be associated with PDR occurrence and in turn regulates VEGF expression among PDR subjects. Springer Science+Business Media New York, 2015 Proliferative diabetic retinopathy nationallicence Non-proliferative diabetic retinopathy nationallicence Nuclear factor kappa B nationallicence Vascular endothelial growth factor nationallicence Reactive oxygen species nationallicence Single nucleotide polymorphisms nationallicence Choudhuri Subhadip Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut Chowdhury Imran Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut Das Shibali Department of Molecular Medicine, Bose Institute, CIT road, 700054, Kolkata, India aut Dutta Deep Department of Endocrinology & Metabolism, IPGMER & SSKM Hospital, 244 AJC Bose Road, 700020, Kolkata, India aut Saha Avijit Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut Sarkar Rajarshi Department of Laboratory Services, Drs. Tribedi and Roy Diagnostic Lab, Park Street, 700016, Kolkata, India aut Mandal Lakshmi Regional Institute of Ophthalmology, 88, College Street, 700088, Kolkata, India aut Mukherjee Satinath Department of Endocrinology & Metabolism, IPGMER & SSKM Hospital, 244 AJC Bose Road, 700020, Kolkata, India aut Bhattacharya Basudev Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 265-279 0300-8177 405:1-2<265 2015 405 11010 https://doi.org/10.1007/s11010-015-2417-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2417-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choudhuri Subhadip Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Chowdhury Imran Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Das Shibali Department of Molecular Medicine, Bose Institute, CIT road, 700054, Kolkata, India aut NATIONALLICENCE 700 1- Dutta Deep Department of Endocrinology & Metabolism, IPGMER & SSKM Hospital, 244 AJC Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Saha Avijit Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Sarkar Rajarshi Department of Laboratory Services, Drs. Tribedi and Roy Diagnostic Lab, Park Street, 700016, Kolkata, India aut NATIONALLICENCE 700 1- Mandal Lakshmi Regional Institute of Ophthalmology, 88, College Street, 700088, Kolkata, India aut NATIONALLICENCE 700 1- Mukherjee Satinath Department of Endocrinology & Metabolism, IPGMER & SSKM Hospital, 244 AJC Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 700 1- Bhattacharya Basudev Department of Biochemistry, Dr. B C Roy Post Graduate Institute of Basic Medical Education and Research (IPGME&R), 244B, A.J.C Bose Road, 700020, Kolkata, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 265-279 0300-8177 405:1-2<265 2015 405 11010