caa a22 4500 606234527 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2401-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2401-7 Effect of microRNA-203 on tumor growth in human hypopharyngeal squamous cell carcinoma [Elektronische Daten] [Ru Wang, Jugao Fang, Hongzhi Ma, Lin Feng, Meng Lian, Fan Yang, Haizhou Wang, Qi Wang, Xiaohong Chen] MicroRNAs (MiRNAs) have been recognized to regulate cancer initiation and progression in carcinogenesis as either oncogenes or tumor suppressor genes, but their role in hypopharyngeal cancer development is not clearly defined. To determine whether miRNA-203 can promote tumor growth in human hypopharyngeal squamous cell carcinoma, we conducted experiments on the functional study of miRNA-203 and identification of miRNA-203 regulated target genes in hypopharyngeal cancer cells. We found that cell proliferation and cell colony-forming increased more in the miRNA-203 up-regulated cancer cells than in the negative control cancer cells. Up-regulation of miRNA-203 accelerated cell cycle progression in hypopharyngeal cancer cells. TP63 and B3GNT5 mRNAs were identified and validated as targets of miRNA-203. However, transwell assay and wound scratch assay showed that miRNA-203 did not involve in invasion and metastasis in hypopharyngeal cancer cells. According to the results, we conclude that miRNA-203 can promote tumor growth in human hypopharyngeal squamous cell carcinoma. These results provide the convincing evidence for the first time that up-regulation of miRNA-203 contributes to the malignancy of hypopharyngeal squamous cell carcinoma, possibly through down-regulating TP63 and B3GNT5. Springer Science+Business Media New York, 2015 Hypopharyngeal neoplasm nationallicence Hsa-mir-203 nationallicence Cell proliferation nationallicence Cell cycle nationallicence Wang Ru Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Fang Jugao Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Ma Hongzhi Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Feng Lin Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Lian Meng Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Yang Fan Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Wang Haizhou Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Wang Qi Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Chen Xiaohong Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 97-104 0300-8177 405:1-2<97 2015 405 11010 https://doi.org/10.1007/s11010-015-2401-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2401-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Ru Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Fang Jugao Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Ma Hongzhi Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Feng Lin Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Lian Meng Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Yang Fan Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Wang Haizhou Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Wang Qi Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 700 1- Chen Xiaohong Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 97-104 0300-8177 405:1-2<97 2015 405 11010