caa a22 4500 606234543 CHVBK 20210128101237.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2394-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2394-2 Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex [Elektronische Daten] [B. Kaločayová, L. Mézešová, M. Barteková, J. Vlkovičová, V. Jendruchová, N. Vrbjar] Time sequence study was performed to characterize the effects of diabetes mellitus type 1 on properties of the Na, K-ATPase in cerebral cortex derived from normal and streptozotocin (STZ)-diabetic rats of both genders. The samples were excised at varying time intervals of diabetes induced by STZ (65mgkg−1) for 8days, and 8 and 16weeks. Expression of α1-3 isoforms of Na, K-ATPase was not altered in statistically significant level during all stages of diabetes neither in female nor in male rats as revealed from Western blot analysis. Studies of kinetic properties of the enzyme resulted in variations in active number of Na, K-ATPase molecules as well as its qualitative properties. Sixteen-week-old control male rats showed better affinity to substrate as indicated by 13% decrease of K m value. The effect persisted also in males subjected to 8days lasting diabetes; however, in males subjected to 8weeks lasting diabetes, the effect was lost. In 25-week-old rats, the Na, K-ATPase revealed again altered properties in males and females but the mechanism of the variation was different. In females, the number of active molecules of Na, K-ATPase was higher by 32% in controls and by 17% in rats with chronic diabetes when comparing to respective male groups as suggested by increased value of V max. So the properties of Na, K-ATPase in cerebral cortex, playing crucial role in maintaining intracellular homeostasis of Na+ ions, depend on gender, age, and duration of diabetic insult. Springer Science+Business Media New York, 2015 Sodium pump nationallicence Gender difference nationallicence Enzyme kinetics nationallicence Kaločayová B. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Mézešová L. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Barteková M. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Vlkovičová J. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Jendruchová V. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Vrbjar N. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 41-52 0300-8177 405:1-2<41 2015 405 11010 https://doi.org/10.1007/s11010-015-2394-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2394-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaločayová B. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Mézešová L. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Barteková M. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Vlkovičová J. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Jendruchová V. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Vrbjar N. Institute for Heart Research, Slovak Academy of Sciences, Dúbravska cesta 9, 842 33, Bratislava, Slovak Republic aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 41-52 0300-8177 405:1-2<41 2015 405 11010