caa a22 4500 606234608 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2415-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2415-1 Nano-Se attenuates cyclophosphamide-induced pulmonary injury through modulation of oxidative stress and DNA damage in Swiss albino mice [Elektronische Daten] [Arin Bhattacharjee, Abhishek Basu, Jaydip Biswas, Sudin Bhattacharya] Chemotherapy is an integral part of modern day treatment regimen but anticancer drugs fail to demarcate between cancerous and normal cells thereby causing severe form of systemic toxicity. Among which pulmonary toxicity is a dreadful complication developed in cancer patients upon cyclophosphamide (CP) therapy. Oxidative stress, fibrosis, and apoptosis are the major patho-mechanisms involved in CP-induced pulmonary toxicity. In the present study, we have synthesized Nano-Se, nanotechnology-based new form of elemental selenium which has significantly lower toxicity and acceptable bioavailability. In order to meet the need of effective drugs against CP-induced adverse effects, nano selenium (Nano-Se) was tested for its possible protective efficacy on CP-induced pulmonary toxicity and bone marrow toxicity. CP intoxication resulted in structural and functional lung impairment which was revealed by massive histopathological changes. Lung injury was associated with oxidative stress/lipid peroxidation as evident by increased in reactive oxygen species, nitric oxide level, and malondialdehyde (MDA) formation with decreased in level of antioxidants such as reduced glutathione, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase, and catalase. Furthermore, CP at a dose of 25mg/kg b.w. increased pulmonary DNA damage (‘comet tail') and triggered DNA fragmentation and apoptosis in mouse bone marrow cells. On the other hand, Nano-Se at a dose of 2mgSe/kg b.w., significantly inhibited CP-induced DNA damage in bronchoalveolar lavage cells, and decreased the apoptosis and percentage of DNA fragmentation in bone marrow cells and also antagonized the reduction of the activities of antioxidant enzymes and the increase level of MDA. Thus, our results suggest that Nano-Se in pre- and co-administration may serve as a promising preventive strategy against CP-induced pulmonary toxicity. Springer Science+Business Media New York, 2015 Nano-Se nationallicence Cyclophosphamide nationallicence Pulmonary toxicity nationallicence Oxidative stress nationallicence Detoxifying enzymes nationallicence DNA damage nationallicence Bhattacharjee Arin Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut Basu Abhishek Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut Biswas Jaydip Department of Translational Research, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut Bhattacharya Sudin Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 243-256 0300-8177 405:1-2<243 2015 405 11010 https://doi.org/10.1007/s11010-015-2415-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2415-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhattacharjee Arin Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Basu Abhishek Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Biswas Jaydip Department of Translational Research, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Bhattacharya Sudin Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, 700026, Kolkata, West Bengal, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 243-256 0300-8177 405:1-2<243 2015 405 11010