caa a22 4500 606234624 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2402-6 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2402-6 Protective effects of N-acetylcysteine against hyperoxaluria induced mitochondrial dysfunction in male wistar rats [Elektronische Daten] [Minu Sharma, Tanzeer Kaur, S. Singla] The purpose of the present study was to evaluate the nephro-protective potential of N-acetylcysteine against hyperoxaluria-induced renal mitochondrial dysfunction in rats. Nine days dosing of 0.4% ethylene glycol +1% ammonium chloride, developed hyperoxaluria in male wistar rats which resulted in renal injury and dysfunction as supported by increased level of urinary lactate dehydrogenase, calcium, and decreased creatinine clearance. Mitochondrial oxidative strain in hyperoxaluric animals was evident by decreased levels of superoxide dismutase, glutathione peroxidase, glutathione reductase, reduced glutathione, and an increased lipid peroxidation. Declined activities of respiratory chain enzymes and tricarboxylic acid cycle enzymes showed mitochondrial dysfunction in hyperoxaluric animals. N-acetylcysteine (50mg/kg, i.p.), by virtue of its -SH reviving power, was able to increase the glutathione levels and thus decrease the oxidative stress in renal mitochondria. Hence, mitochondrial damage is, evidently, an essential event in ethylene glycol-induced hyperoxaluria and N-acetylcysteine presented itself as a safe and effective remedy in combating nephrolithiasis. Springer Science+Business Media New York, 2015 Mitochondria nationallicence Hyperoxaluria nationallicence Oxidative stress nationallicence N-acetylcysteine nationallicence Vitamin E nationallicence Antioxidant nationallicence COM : Calcium oxalate monohydrate nationallicence DTNB : 5,5-Dithiobis-2-nitrobenzoic acid nationallicence EG : Ethylene glycol nationallicence ETC : Electron transport chain nationallicence GR : Glutathione reductase nationallicence GSH : Reduced glutathione nationallicence GPx : Glutathione peroxidase nationallicence i.p. : Intraperitoneal nationallicence LDH : Lactate dehydrogenase nationallicence LPO : Lipid peroxidation nationallicence NP-SH : Non-protein thiols nationallicence MDA : Malondialdehyde nationallicence Mn-SOD : Manganese superoxide dismutase nationallicence NADH : Nicotinamide adenine dinucleotide nationallicence NAC : N-acetylcysteine nationallicence ROS : Reactive oxygen species nationallicence T-SH : Total thiols nationallicence Sharma Minu Department of Biochemistry, Panjab University, 160014, Chandigarh, India aut Kaur Tanzeer Department of Biophysics, Panjab University, Chandigarh, India aut Singla S. Department of Biochemistry, Panjab University, 160014, Chandigarh, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 105-114 0300-8177 405:1-2<105 2015 405 11010 https://doi.org/10.1007/s11010-015-2402-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2402-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma Minu Department of Biochemistry, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Kaur Tanzeer Department of Biophysics, Panjab University, Chandigarh, India aut NATIONALLICENCE 700 1- Singla S. Department of Biochemistry, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 105-114 0300-8177 405:1-2<105 2015 405 11010