caa a22 4500 606234632 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2413-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2413-3 Transcriptional modulation of mitochondria biogenesis pathway at and above critical speed in mice [Elektronische Daten] [L. Mille-Hamard, C. Breuneval, A. Rousseau, P. Grimaldi, V. Billat] High- or moderate-intensity endurance training leads to mitochondrial biogenesis via the peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α)/mitochondrial transcription factor A (Tfam) signaling pathway. Although this pathway is stimulated during acute exercise, the relationship between its activity and the intensity of the exercise has not been characterized. In animal studies, individualized running speeds have not previously been assessed. Here, we sought to determine whether this pathway was modulated after a bout of exhaustive exercise at different relative intensities (at and over critical speed (CS)). Our starting hypotheses were that (i) exercise-induced overexpression of PGC-1α in skeletal muscle falls at intensities above CS, and (ii) transcriptional activity of the mitochondrial biogenesis signaling cascade is intensity-sensitive at and above CS. To test these hypothesis, male Friend Virus B-Type mice were divided into a control group and three exercise groups (exercising at CS, peak velocity (vPeak) and 150% CS, respectively). mRNA expression levels for genes involved in mitochondrial biogenesis signaling were analyzed in the quadriceps muscle. PGC-1α was overexpressed at all exercise intensities. We also identified that, PGC-1α mRNA expression was negatively correlated with exercise intensity and blood lactate levels but not with maximal oxygen uptake, vPeak, or CS. Expression of the PGC-1α co-activator peroxisome proliferator-activated receptor β was negatively correlated with the exercise intensity. In contrast, expression levels of Tfam were dissociated from exercise intensity. Our data indicate that at the intensities used in endurance training, the expression of mitochondrial biogenesis genes is finely modulated by the relative intensity of exhaustive exercise. Springer Science+Business Media New York, 2015 Exercise nationallicence Muscle nationallicence Mitochondria biogenesis nationallicence $$ {\dot{\text{V}}\text{O}}_{{ 2 {\text{max}}}} $$ V ˙ O 2 max : Maximal oxygen uptake nationallicence PGC-1α : Peroxisome proliferator-activated receptor-γ coactivator-1α nationallicence CS : Critical speed nationallicence PPARβ : Peroxisome proliferator-activated receptor beta nationallicence Tfam : Mitochondrial transcription factor A nationallicence Sirt-1 : Sirtuin 1 nationallicence VO2 : Oxygen consumption nationallicence vPeak : Peak velocity nationallicence [La] rest : Blood lactate concentration at rest nationallicence C t : Critical threshold nationallicence AMPK : AMP-activated protein kinase nationallicence miRNA : microRNA nationallicence Mille-Hamard L. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut Breuneval C. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut Rousseau A. C3M, INSERM 1065, Université Nice-Sophia-Antipolis, Nice, France aut Grimaldi P. C3M, INSERM 1065, Université Nice-Sophia-Antipolis, Nice, France aut Billat V. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 223-232 0300-8177 405:1-2<223 2015 405 11010 https://doi.org/10.1007/s11010-015-2413-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2413-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mille-Hamard L. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut NATIONALLICENCE 700 1- Breuneval C. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut NATIONALLICENCE 700 1- Rousseau A. C3M, INSERM 1065, Université Nice-Sophia-Antipolis, Nice, France aut NATIONALLICENCE 700 1- Grimaldi P. C3M, INSERM 1065, Université Nice-Sophia-Antipolis, Nice, France aut NATIONALLICENCE 700 1- Billat V. UBIAE, INSERM U902, Université Evry-Val d'Essonne, Bd Francois Mitterrand, Batiment Maupertuis, 91025, Evry, France aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 223-232 0300-8177 405:1-2<223 2015 405 11010