caa a22 4500 606234640 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11010-015-2399-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2399-x Effects of delayed motherhood on hippocampal gene expression in offspring rats [Elektronische Daten] [Ping Duan, Bo Li, Caifang Li, Xuefei Han, Yan Xu, Ying Xing, Wenhai Yan] While many studies have examined the pregnancy and health-related outcomes of delayed motherhood for women, less is known concerning the potential consequences for their children. This study aims to investigate the effect of delayed motherhood on the hippocampus at the whole genome level. Sprague-Dawley rat females, either at the age of 3 or 12months, were individually housed with a randomly selected 3-month-old male. The rat whole genome expression chips were used to detect gene expression differences in the hippocampus of newborn rats. The gene expression profile was studied through gene ontology and signal pathway analyses. qRT-PCR was used to determine the mRNA expression of solute carrier family 2 (SLC2A1) and S-phase kinase-associated protein 2 (SKP2). Western blot was used to detect the protein expression of SKP2. Compared to the control group, 1291 differentially expressed genes were detected, including 635 up-regulated genes and 656 down-regulated genes. These differential expressed genes were involved in 110 significant biological process and nine significant signaling pathways, in which the pathway in cancer is the most changed pathway. For SKP2 (up-regulated) and SLC2A1 (up-regulated) genes which were relevant to the pathway in cancer, qRT-PCR results were consistent with gene chip assay results. The upregulation of SKP2 was also demonstrated at protein level. In conclusion, delayed motherhood led to unique patterns of hippocampal gene expression in offspring and the newly identified genes afford a quantitative view of the changes which enable deeper insights into the molecular basis underlying the role of delayed motherhood. Springer Science+Business Media New York, 2015 Delayed motherhood nationallicence Hippocampus nationallicence Microarray nationallicence SKP2 nationallicence SLC2A1 nationallicence Duan Ping Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Li Bo Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Li Caifang Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Han Xuefei Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Xu Yan Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Xing Ying Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Yan Wenhai Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 89-95 0300-8177 405:1-2<89 2015 405 11010 https://doi.org/10.1007/s11010-015-2399-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2399-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duan Ping Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Li Bo Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Li Caifang Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Han Xuefei Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Xu Yan Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Xing Ying Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Yan Wenhai Department of Basic Medicine, Institute of Basic Medicine, Zhengzhou University, Zhengzhou, Henan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 405/1-2(2015-07-01), 89-95 0300-8177 405:1-2<89 2015 405 11010