caa a22 4500 606234683 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2524-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2524-x Reduction of oxidative stress by an ethanolic extract of leaves of Piper betle (Paan) Linn. decreased methotrexate-induced toxicity [Elektronische Daten] [Soumita De, Tuhinadri Sen, Mitali Chatterjee] Methotrexate (MTX), a folate antagonist, is currently used as first line therapy for autoimmune diseases like rheumatoid arthritis and psoriasis, but its use is limited by the associated hepatotoxicity. As leaves of Piper betle, belonging to family Piperaceae, have antioxidant and anti-inflammatory properties, the present study was undertaken to investigate the potential of Piper betle leaf extract (PB) in attenuating MTX-induced hepatotoxicity. Rats pre-treated with PB (50 or 100mgkg−1 b.w., p.o.) were administered with a single dose of MTX (20mgkg−1, b.w., i.p.) and its hepatoprotective efficacy was compared with folic acid (1mgkg−1 b.w., i.p.), conventionally used to minimize MTX-induced toxicity. MTX-induced hepatotoxicity was confirmed by increased activities of marker enzymes, alanine transaminase, aspartate transaminase, and alkaline phosphatase which were remitted by pre-treatment with PB and corroborated with histopathology. Additionally, MTX-induced hepatic oxidative stress which included increased generation of reactive oxygen species, enhanced lipid peroxidation, depleted levels of glutathione and decreased activities of antioxidant enzymes was effectively mitigated by PB, indicative that its promising antioxidant-mediated hepatoprotective activity was worthy of future pharmacological consideration. Springer Science+Business Media New York, 2015 Antioxidant nationallicence Hepatoprotective nationallicence Methotrexate nationallicence Piper betle nationallicence De Soumita Department of Pharmacology, Institute of Postgraduate Medical Education and Research, 244 B, Acharya JC Bose Road, 700020, Kolkata, West Bengal, India aut Sen Tuhinadri Department of Pharmaceutical Technology, Jadavpur University, 188, Raja Subodh Chandra Mullick Road, Jadavpur, 700032, Kolkata, West Bengal, India aut Chatterjee Mitali Department of Pharmacology, Institute of Postgraduate Medical Education and Research, 244 B, Acharya JC Bose Road, 700020, Kolkata, West Bengal, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 191-197 0300-8177 409:1-2<191 2015 409 11010 https://doi.org/10.1007/s11010-015-2524-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2524-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Soumita Department of Pharmacology, Institute of Postgraduate Medical Education and Research, 244 B, Acharya JC Bose Road, 700020, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Sen Tuhinadri Department of Pharmaceutical Technology, Jadavpur University, 188, Raja Subodh Chandra Mullick Road, Jadavpur, 700032, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Chatterjee Mitali Department of Pharmacology, Institute of Postgraduate Medical Education and Research, 244 B, Acharya JC Bose Road, 700020, Kolkata, West Bengal, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 191-197 0300-8177 409:1-2<191 2015 409 11010