caa a22 4500 606234691 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2517-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2517-9 Bergapten promotes bone marrow stromal cell differentiation into osteoblasts in vitro and in vivo [Elektronische Daten] [Ji-jie Xiao, Wen-ji Zhao, Xin-tao Zhang, Wen-long Zhao, Xiao-xia Wang, Shu-hui Yin, Fang Jiang, Yin-xia Zhao, Fang-ni Chen, Shao-lin Li] Many recent studies have suggested that bergapten (BP), a class of native compound with numerous biological activities such as anti-resorptive properties, may exert protective effects against postmenopausal bone loss. However, it remains unknown whether BP regulates or improves the osteogenic function of bone marrow stromal cells (BMSCs) in the treatment and prevention of osteoporosis. In our study, BMSCs were cultured in osteogenic induction medium with the addition of BP for 2weeks and an ovariectomized mouse model of osteoporosis was used to investigate the anti-resorptive effect of BP by gavage administration for 3months. The concentrations of BP used were 0.1, 1, and 10μmol/L in vitro and the gavage dose was 20mg/kg/d. The result of our study indicated that BP promotes the expression of alkaline phosphatase (ALP) by BMSCs in vitro in a dose-dependent manner, as revealed by ALP staining. Runt-related transcription factor 2 and osteocalcin were up-regulated both in vitro and vivo, while osterix and collagen Iα1, assessed by immunofluorescence and immunohistochemistry, were correspondingly raised in the presence of BP in BMSCs in vitro. In addition, a protective effect of BP against ovariectomy-induced bone loss was found by distal femur micro-CT scanning, with improvements of bone metabolism parameters such as bone mineral density, trabecular number, and trabecular separation. Furthermore, WNT/β-catenin signaling was activated in the presence of BP in BMSCs in osteogenic culture. Finally, BP promoted differentiation of BMSCs into osteoblasts by up-regulation of the WNT/β-catenin pathway. Graphical abstract: Springer Science+Business Media New York, 2015 Bergapten nationallicence Osteoblast nationallicence BMSC nationallicence Osteoporosis nationallicence Bone formation nationallicence Xiao Ji-jie Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Zhao Wen-ji Department of Medical Image, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut Zhang Xin-tao Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Zhao Wen-long Clinical Laboratory, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut Wang Xiao-xia Endocrinology Department, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut Yin Shu-hui Clinical Laboratory, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut Jiang Fang Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Zhao Yin-xia Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Chen Fang-ni Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Li Shao-lin Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 113-122 0300-8177 409:1-2<113 2015 409 11010 https://doi.org/10.1007/s11010-015-2517-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2517-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Ji-jie Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 700 1- Zhao Wen-ji Department of Medical Image, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut NATIONALLICENCE 700 1- Zhang Xin-tao Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 700 1- Zhao Wen-long Clinical Laboratory, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut NATIONALLICENCE 700 1- Wang Xiao-xia Endocrinology Department, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut NATIONALLICENCE 700 1- Yin Shu-hui Clinical Laboratory, Inner Mongolia Bayannaoer City Hospital, 015000, Bayannaoer City, China aut NATIONALLICENCE 700 1- Jiang Fang Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 700 1- Zhao Yin-xia Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 700 1- Chen Fang-ni Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 700 1- Li Shao-lin Department of Medical Image, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, 510630, Guangzhou, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 113-122 0300-8177 409:1-2<113 2015 409 11010