caa a22 4500 606234713 CHVBK 20210128101238.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2514-z doi (NATIONALLICENCE)springer-10.1007/s11010-015-2514-z The role of PPARγ in TBBPA-mediated endocrine disrupting effects in human choriocarcinoma JEG-3 cells [Elektronische Daten] [Ewelina Honkisz, Anna Wójtowicz] The goal of the present study was to investigate the action of TBBPA on PPARγ protein expression in vitro in human choriocarcinoma-derived placental JEG-3 cells. We also analyzed TBBPA for its action on placental secretion of progesterone and β-hCG, cell viability, and apoptosis. Our results showed that after TBBPA treatment at 10nM and 10µM, PPARγ protein expression increased in a time-dependent manner until 48h and then slightly decreased at 72h but was still above the control level. This alteration in PPARγ protein expression was accompanied by a decreased β-hCG level. Interestingly, co-treatment with the PPARγ antagonist GW9662 reversed the TBBPA-mediated changes in PPARγ protein expression but, according to β-hCG secretion, potentiated an inhibitory effect of TBBPA. Additionally, in our study, we assessed the ability of TBBPA to increase progesterone levels in JEG-3 cells compared with those of controls. Finally, in the present study, we demonstrated that TBBPA at all of the tested doses significantly increased caspase-3 activity compared with that of the vehicle control. The apoptotic action of TBBPA was also confirmed by Hoechst 33342 staining. These results showed the up-regulation of PPARγ protein expression after TBBPA exposure in human placental cells. Although co-treatment with antagonist of PPARγ reversed the TBBPA-mediated increase in this protein expression and restored it to the control level, it did not reverse the effect on β-hCG secretion. This indicated that the mechanism of TBBPA-induced changes in β-hCG secretion is PPARγ-independent. The Author(s), 2015 Tetrabromobisphenol A nationallicence Peroxisome proliferator-activated receptor gamma nationallicence β-hCG nationallicence Progesterone nationallicence Caspase-3 nationallicence JEG-3 nationallicence Honkisz Ewelina Department of Animal Biotechnology, University of Agriculture, Rędzina 1B, 30-248, Kraków, Poland aut Wójtowicz Anna Department of Animal Biotechnology, University of Agriculture, Rędzina 1B, 30-248, Kraków, Poland aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 81-91 0300-8177 409:1-2<81 2015 409 11010 https://doi.org/10.1007/s11010-015-2514-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2514-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Honkisz Ewelina Department of Animal Biotechnology, University of Agriculture, Rędzina 1B, 30-248, Kraków, Poland aut NATIONALLICENCE 700 1- Wójtowicz Anna Department of Animal Biotechnology, University of Agriculture, Rędzina 1B, 30-248, Kraków, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 81-91 0300-8177 409:1-2<81 2015 409 11010