caa a22 4500 606234861 CHVBK 20210128101239.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2523-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2523-y Glycyrrhizin ameliorates metabolic syndrome-induced liver damage in experimental rat model [Elektronische Daten] [Rajarshi Sil, Doel Ray, Abhay Chakraborti] Glycyrrhizin, a major constituent of licorice (Glycyrrhiza glabra) root, has been reported to ameliorate insulin resistance, hyperglycemia, dyslipidemia, and obesity in rats with metabolic syndrome. Liver dysfunction is associated with this syndrome. The objective of this study is to investigate the effect of glycyrrhizin treatment on metabolic syndrome-induced liver damage. After induction of metabolic syndrome in rats by high fructose (60%) diet for 6weeks, the rats were treated with glycyrrhizin (50mg/kg body weight, single intra-peritoneal injection). After 2weeks of treatment, rats were sacrificed to collect blood samples and liver tissues. Compared to normal, elevated activities of serum alanine transaminase, alkaline phosphatase and aspartate transaminase, increased levels of liver advanced glycation end products, reactive oxygen species, lipid peroxidation, protein carbonyl, protein kinase Cα, NADPH oxidase-2, and decreased glutathione cycle components established liver damage and oxidative stress in fructose-fed rats. Activation of nuclear factor κB, mitogen-activated protein kinase pathways as well as signals from mitochondria were found to be involved in liver cell apoptosis. Increased levels of cyclooxygenase-2, tumor necrosis factor, and interleukin-12 proteins suggested hepatic inflammation. Metabolic syndrome caused hepatic DNA damage and poly-ADP ribose polymerase cleavage. Fluorescence-activated cell sorting using annexin V/propidium iodide staining confirmed the apoptotic hepatic cell death. Histology of liver tissue also supported the experimental findings. Treatment with glycyrrhizin reduced oxidative stress, hepatic inflammation, and apoptotic cell death in fructose-fed rats. The results suggest that glycyrrhizin possesses therapeutic potential against hepatocellular damage in metabolic syndrome. Springer Science+Business Media New York, 2015 Metabolic syndrome nationallicence Glycyrrhizin nationallicence Liver damage nationallicence Oxidative stress nationallicence Apoptosis nationallicence Inflammation nationallicence Sil Rajarshi Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut Ray Doel Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut Chakraborti Abhay Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 177-189 0300-8177 409:1-2<177 2015 409 11010 https://doi.org/10.1007/s11010-015-2523-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2523-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sil Rajarshi Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Ray Doel Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Chakraborti Abhay Department of Biophysics, Molecular Biology and Bioinformatics, University College of Science, University of Calcutta, 92 Acharyya Prafulla Chandra Road, 700009, Kolkata, West Bengal, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 177-189 0300-8177 409:1-2<177 2015 409 11010