caa a22 4500 60623487X CHVBK 20210128101239.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2520-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2520-1 Oleanolic acid alleviated pressure overload-induced cardiac remodeling [Elektronische Daten] [Hai-Han Liao, Nan Zhang, Hong Feng, Ning Zhang, Zhen-Guo Ma, Zheng Yang, Yuan Yuan, Zhou-Yan Bian, Qi-Zhu Tang] Previous study has demonstrated that oleanolic acid (OA) possessing the anti-inflammatory and anti-oxidant properties blunted high-glucose-induced diabetic cardiomyopathy and ameliorated experimental autoimmune myocarditis in mice. However, little is known about its effects on pressure overload-induced cardiac remodeling. Herein, we investigated the effect of OA on cardiac remodeling and underlying mechanism. Mice, subjected to aortic banding (AB), were randomly assigned into control group and experimental group. OA premixed in diets was administered to mice after 3days of AB. Echocardiography and catheter-based measurements of hemodynamic parameters were performed after 8weeks' treatment of OA. Histologic examination and molecular analyses were used to assess cardiac hypertrophy and tissue fibrosis. In addition, the inhibitory effects of OA on H9c2 cardiomyocytes and cardiac primary fibroblast responded to the stimulation of AngII were also investigated. OA ameliorated the systolic and diastolic dysfunction induced by pressure overload evidenced by echocardiography and catheter-based measurements. OA also decreased the mRNA expression of cardiac hypertrophy and fibrosis markers evidenced by RT-PCR. It has been shown in our study that pressure overload activated the phosphorylations of Akt, mTOR, p70s6k, S6, GSK3β, and FoxO3a, and treatment of OA attenuated the phosphorylation of these proteins. In addition, hypertrophy of cardiomyocytes and fibrosis markers induced by AngII was inhibited by OA in vitro. Our findings uncover that OA suppressed AB-induced cardiac hypertrophy, partly by inhibiting the activity of Akt/mTOR pathway, and suggest that treatment of OA may have a benefit on retarding the progress of cardiac remodeling under long terms of pressure overload. Springer Science+Business Media New York, 2015 Oleanolic acid nationallicence Cardiac hypertrophy nationallicence Fibrosis nationallicence Akt/mTOR nationallicence Liao Hai-Han Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Zhang Nan Renmin Hospital of Wuhan University, Wuhan, China aut Feng Hong Zhong Nan Hospital of Wuhan University, Wuhan, China aut Zhang Ning Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Ma Zhen-Guo Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Yang Zheng Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Yuan Yuan Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Bian Zhou-Yan Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Tang Qi-Zhu Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 145-154 0300-8177 409:1-2<145 2015 409 11010 https://doi.org/10.1007/s11010-015-2520-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2520-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Hai-Han Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Nan Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Feng Hong Zhong Nan Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Ning Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Ma Zhen-Guo Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Yang Zheng Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Yuan Yuan Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Bian Zhou-Yan Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Tang Qi-Zhu Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 145-154 0300-8177 409:1-2<145 2015 409 11010