caa a22 4500 60623490X CHVBK 20210128101239.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2512-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2512-1 Sumoylated α -skeletal muscle actin in the skeletal muscle of adult rats [Elektronische Daten] [Munehiro Uda, Hiroaki Kawasaki, Kyoichi Iizumi, Ayako Shigenaga, Takeshi Baba, Hisashi Naito, Toshitada Yoshioka, Fumiyuki Yamakura] Skeletal muscles are composed of two major muscle fiber types: slow-twitch oxidative fibers and fast-twitch glycolytic fibers. The proteins in these muscle fibers are known to differ in their expression, relative abundance, and post-translational modifications. In this study, we report a previously unreported post-translational modification of α-skeletal muscle actin in the skeletal muscles of adult male F344 rats in vivo. Using two-dimensional electrophoresis (2D-PAGE), we first examined the differences in the protein expression profiles between the soleus and plantaris muscles. We found higher intensity protein spots at approximately 60kDa and pH 9 on 2D-PAGE for the soleus muscle compared with the plantaris muscle. These spots were identified as α-skeletal muscle actin by liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry and western blot analyses. In addition, we found that the 60kDa α-skeletal muscle actin is modified by small ubiquitin-like modifier (SUMO) 1, using 2D-PAGE and western blot analyses. Furthermore, we found that α-skeletal muscle actin with larger molecular weight was localized in the nuclear and cytosol of the skeletal muscle, but not in the myofibrillar fraction by the combination of subcellular fractionation and western blot analyses. These results suggest that α-skeletal muscle actin is modified by SUMO-1 in the skeletal muscles, localized in nuclear and cytosolic fractions, and the extent of this modification is much higher in the slow muscles than in the fast muscles. This is the first study to show the presence of SUMOylated actin in animal tissues. Springer Science+Business Media New York, 2015 Skeletal muscles nationallicence Fiber-type composition nationallicence SUMOylation nationallicence Nuclear actin nationallicence EDL : Extensor digitorum longus nationallicence IEF : Isoelectric focusing nationallicence IPG : Immobilized pH gradient nationallicence LC-ESI-MS-MS : Liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry nationallicence SUMO-1, 2, or 3 : Small ubiquitin-like modifier 1, 2, or 3 nationallicence 2D-PAGE : Two-dimensional electrophoresis nationallicence Uda Munehiro Sportology Center, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Japan aut Kawasaki Hiroaki The Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Inzai, Japan aut Iizumi Kyoichi The Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Inzai, Japan aut Shigenaga Ayako Institute of Health and Sports Science & Medicine, Juntendo University Graduate School of Health and Sports Science, Inzai, Japan aut Baba Takeshi Juntendo University School of Medicine, Inzai, Japan aut Naito Hisashi Institute of Health and Sports Science & Medicine, Juntendo University Graduate School of Health and Sports Science, Inzai, Japan aut Yoshioka Toshitada Faculty of Social Welfare, Hirosaki Gakuin University, Hirosaki, Japan aut Yamakura Fumiyuki Department of Chemistry, Juntendo University School of Health Care and Nursing, Inzai, Japan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 59-66 0300-8177 409:1-2<59 2015 409 11010 https://doi.org/10.1007/s11010-015-2512-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2512-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uda Munehiro Sportology Center, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Japan aut NATIONALLICENCE 700 1- Kawasaki Hiroaki The Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Inzai, Japan aut NATIONALLICENCE 700 1- Iizumi Kyoichi The Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Inzai, Japan aut NATIONALLICENCE 700 1- Shigenaga Ayako Institute of Health and Sports Science & Medicine, Juntendo University Graduate School of Health and Sports Science, Inzai, Japan aut NATIONALLICENCE 700 1- Baba Takeshi Juntendo University School of Medicine, Inzai, Japan aut NATIONALLICENCE 700 1- Naito Hisashi Institute of Health and Sports Science & Medicine, Juntendo University Graduate School of Health and Sports Science, Inzai, Japan aut NATIONALLICENCE 700 1- Yoshioka Toshitada Faculty of Social Welfare, Hirosaki Gakuin University, Hirosaki, Japan aut NATIONALLICENCE 700 1- Yamakura Fumiyuki Department of Chemistry, Juntendo University School of Health Care and Nursing, Inzai, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 59-66 0300-8177 409:1-2<59 2015 409 11010